高效液相色谱法测定橘红枇杷片中柚皮苷的含量
Determination of Naringin in Juhong Pipa Tablets by HPLC
张燕1
作者信息
- 1. 楚雄技师学院,云南 楚雄 675000
- 折叠
摘要
(目的)建立用高效液相色谱法测定橘红枇杷片中柚皮苷含量的方法;(方法)除去橘红枇杷片糖衣后,研细,加入甲醇,加热回流,放冷,补足损失质量,过滤,取滤液进样.色谱仪为安捷伦 HP-1100 型,色谱柱为Agilent ZORBAX C18柱,紫外测定检测波长选定为283 nm,流动相为乙腈-0.1%磷酸(体积比20∶80),流速为1 mL/min.结果 表明,在柚皮苷质量浓度为 7.237～115.8 μg/mL 范围内,线性关系良好(r =0.99994,n =6),方法回收率为98.68%(n =6).(结论)本方法准确、灵敏度高,重现性好,可作为橘红枇杷片质量控制的检测方法.
Abstract
Objective:To establish a HPLC method for the determination of naringin in Juhong Pipa tablets.Method:To remove the sugar coat-ing of Juhong Pipa tablets,add methanol,heat,reflux,cool,make up weight loss,filter,and continue filtrate for sampling.The chromato-graphwas Agilent HP-1100 column,Agilent ZORBAX C18 column,mobile phase,acetonitrile-0.1%phosphoric acid(20∶80,V/V),flow rate was1 mL/min,and ultraviolet detection wavelength was283 nm.Results:The linear relationship was good in the range of 0.072-1.16 μg(r =0.99994 n =6)and the recovery rate was 98.68%(n =6).Conclusion:This method has high accuracy,sensitivity and good repro-ducibility,and can be used for the control content determination of naringin in Juhong Pipa tablets.
关键词
高效液相色谱法/柚皮苷含量/橘红枇杷片Key words
Juhong pipa tablets/naringin/HPLC引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据