Study on the Fingerprint of Ethnic Medicine Erigeron Breviscapusand its Adulterants
The differences between erigeron breviscapus and its adulterants in different medicinal parts by UPLC fingerprint were com-pared to provide a basis for the identification and utilization of erigeron breviscapus by combining similarity analysis.An agilent ZORBAX E-clipse Plius C18(ϕ2.1 mm×100 mm,1.8 μm)chromatographic column was used,the mobile phase was water(containing 0.1%formic acid)and acetonitrile(containing 0.1%formic acid)with gradient elution,the flow rate was 0.2 mL/min and the detection wavelength was 300 nm.A total of 19 common peaks were calibrated in the established fingerprint.Comparing with the fingerprints,there were no significant differences in the stems,leaves and whole herb of erigeron breviscapus.Nevertheless,discrepancy was observed in fingerprint of flower.The established fin-gerprint method could identify erigeron breviscapusand its adulterants.The similarity was high in erigeron breviscapus,erigeron patentisquama and erigeron multiradiatus.Meanwhile,the similarity of flower was high in erigeron breviscapus,erigeron patentisquama,erigeron multiradiatus,Aster farreriand erigeron annuus.This study is helpful for the identification and utilization of erigeron breviscapus,and can provide reference for the quality control and development of erigeron breviscapus.
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