Determination of 10 Sweeteners in E-cigarette Liquid by HPLC-ELSD
The content of acesulfame,saccharin sodium,sodium cyclamate,sucralose,aspartame,alitame,neomethyl hesperidin dihydrochalcone(NHDC),adevant,mogroside V and neotame in e-cigarette liquid was simultaneously detected by HPLC-ELSD.The sweeteners in the sam-ples were extracted with 50%methanol in water,separated on a C18 column,and quantified by external standard method with a gradient elution using methanol-0.1%formic acid in water(pH =4.5)as mobile phase.The results showed of 10 sweeteners were effectively separated.A quadratic curve was established at 5-250 mg/L,with R2 greater than 0.998.The recovery rate of the method is 90.0%~110.0%,and the RSD is 0.774%~7.65%.The method is simple,accurate and sensitive for the determination of 10 sweeteners in e-cigarette liquids,which is practi-cal and improves the efficiency of laboratory testing.
E-liquidsweetenershigh performance liquid chromatographyevaporative light scattering test