首页|HPLC-ELSD法同时测定电子烟烟液中的10种甜味剂

HPLC-ELSD法同时测定电子烟烟液中的10种甜味剂

扫码查看
建立了高效液相色谱仪-蒸发光散射检测器(HPLC-ELSD)同时检测电子烟烟液中安赛蜜、糖精钠、甜蜜素、三氯蔗糖、阿斯巴甜、阿力甜、新甲基橙皮苷二氢查尔酮(NHDC)、爱德万甜、罗汉果苷V、纽甜的方法.样品中的甜味剂经50%甲醇水提取后,采用 C18色谱柱分离,以甲醇-0.1%甲酸水(pH =4.5)为流动相进行梯度洗脱,蒸发光散射检测器进行检测.结果表明,10 种甜味剂分离度良好,在5~250 mg/L建立二次曲线,R2 均大于0.998,方法回收率为90.0%~110.0%,RSD 为 0.774%~7.65%.检测方法简单、准确、灵敏、实用性强,适用于电子烟烟液中10 种甜味剂的同时检测,提高了实验室的检测效率.
Determination of 10 Sweeteners in E-cigarette Liquid by HPLC-ELSD
The content of acesulfame,saccharin sodium,sodium cyclamate,sucralose,aspartame,alitame,neomethyl hesperidin dihydrochalcone(NHDC),adevant,mogroside V and neotame in e-cigarette liquid was simultaneously detected by HPLC-ELSD.The sweeteners in the sam-ples were extracted with 50%methanol in water,separated on a C18 column,and quantified by external standard method with a gradient elution using methanol-0.1%formic acid in water(pH =4.5)as mobile phase.The results showed of 10 sweeteners were effectively separated.A quadratic curve was established at 5-250 mg/L,with R2 greater than 0.998.The recovery rate of the method is 90.0%~110.0%,and the RSD is 0.774%~7.65%.The method is simple,accurate and sensitive for the determination of 10 sweeteners in e-cigarette liquids,which is practi-cal and improves the efficiency of laboratory testing.

E-liquidsweetenershigh performance liquid chromatographyevaporative light scattering test

叶智莲、杨文武、况利平

展开 >

深圳市吉迩科技有限公司喜马拉雅实验室, 广东 深圳 518000

电子烟烟液 甜味剂 高效液相色谱法 蒸发光散射检测

2024

云南化工
云南省化工研究院 云天化集团有限责任公司 云南煤化工集团有限公司 云南省化学化工学会

云南化工

影响因子:0.272
ISSN:1004-275X
年,卷(期):2024.51(3)
  • 12