Analysis of Protocatechuic Aldehyde in Yindan Xintai Dropping Pills of Medicinal Herbs by HPLC-DAD
(Objective)To establish a method for determination of protocatechuic aldehyde in Yindan xintai dropping pills.(Methods)HPLC-DAD method was used.The chromatographic column was Agilent Eclipse Plus C18(5 μm,250 mm × ϕ4.6 mm).The mobile phase was 0.1％ phosphoric acid solution-methanol(85:15,V/V).The flow rate was 1.0 mL/min;the column temperature was 30 ℃;the detection wavelength was 278 nm;and the injection volume was 10 μL.(Results)The mass concentration of protocatechuic aldehyde showed a good linear relationship between the mass concentration(8.2995～41.4973 μg/mL)and peak area(r=0.9999),with an average recovery rate of 101.1％(RSD:1.5％,n=6).(Conclusion)This quantitative method is accurate and reliable with good reproducibility,which can be used for quality control of Yindan xintai dropping pills.
Protocatechuic aldehydeYindan Xintai dropping pillsHPLC-DADQuality control
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