Simultaneous Determination of Two Volatile Components in Changshu Zhixie Preparations by GC
(Objective)To establish a gas chromatography method for the simultaneous determination of bornyl acetate and dehydrocostus lactone in Changshu Zhixie preparations.(Methods)HP-INNOWax capillary column(30 m × ϕ0.32 mm,0.25 μm)was used as FID detector.The inlet temperature was 240.0 ℃,and the detector temperature was 270.0 ℃.The initial column temperature was maintained at 150 ℃ keep in 10 min,then increased to 250 ℃ at 20 ℃/min keep in 20 min.The gas was helium with a flow rate of 1 mL/min.The injection method was split injection with a split ratio of 10:1.The injection volume was 1 μL,and the air flow rate was 400 mL/min.The flow rate of hydrogen was 30 mL/min.The tail blowing flowrate was 25 mL/min.(Results)The linear ranges of bornyl acetate and dehydrocostus lactone were 3.287～525.893 μg/mL(r=0.9998)and 2.709～433.4423 μg/mL(r=0.9999)respectively,and the linear ranges were good in this range.The average recoveries were 94.13％ and 96.20％,RSD were 1.84％ and 1.36％ respectively.(Conclusion)A gas chromatography method for simultaneous determination of bornyl acetate and dehydrocostus lactone in Changshu Zhixie preparations was established.The method is effective and reliable,and can be used to evaluate the quality control of Changshu Zhixie preparations.
Gas chromatographyChangshu Zhixie preparationsDehydrocostus lactoneBornyl acetate
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