Study on the Degradation Characteristics of DEHP by Bacillus Cereus
This study selected DEHP as the target pollutant and used DEHP degrading bacterium X41,which was enriched and domesticated from the rhizosphere soil of Typha Linn,a plant in the Jiuxi artificial wetland,as the test strain.By analyzing its morphology,physiological and bio-chemical characteristics,and 16S rDNA sequence,the strain X41 was preliminarily identified as Bacillus cereus.Orthogonal experiments were used to study the optimal degradation conditions and characteristics of strain X41 on DEHP.The results showed that strain X41 had the highest degradation efficiency under environmental conditions of pH 6.0,temperature 35℃,inoculation rate 3%,and initial DEHP concentration of 300 mg·L-1.And under this optimal degradation condition,strain X41 grows well,with a growth curve in an"S"shape,and a 7-day DEHP degradation rate of 99.54%.The results of this experiment can provide a certain scientific basis for the removal of DEHP in artificial wetlands.
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