Study on the Growth and Enzyme Production Characteristics of Several Bacillus Strains
This study conducted tolerance tests on several strains of Bacillus stored in the laboratory and explored their enzyme production condi-tions.The results showed that the optimal pH range for Bacillus growth was6.0-8.0,the optimal growth temperature was35℃,and the optimal cultivation speed was 150-180 rpm.The optimal water condition for the growth of the strain in solid-state culture medium is 35%.When the water content isgreater than35%,the protease activity ishigher,and the highest protease activity is143.63 U/g.The temperature hasa signifi-cant impact on the growth of Bacillus,with better growth in the range of 35-45℃.The optimal temperature for protease production is 40℃.A-cidity has a significant impact on the growth of Bacillus.Under pH 5-6 conditions,the strain is inhibited to varying degrees.The strain can pro-duce protease under both acidic and neutral conditions.The orthogonal experiment showed that Bacillus had the highest protease activity under solid-state cultivation conditions of 40%water content,35℃,and pH 8.0.
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