几株芽孢杆菌生长及产酶特性研究
Study on the Growth and Enzyme Production Characteristics of Several Bacillus Strains
吴云昭 1李向广 1尚元贺1
作者信息
- 1. 安图实验仪器 (郑州) 有限公司,河南 郑州 450048
- 折叠
摘要
对实验室保藏的几株芽孢杆菌进行耐受性试验,并探究其产酶条件.结果表明:芽孢杆菌最适生长pH范围为6.0～8.0,最适生长温度为35℃,最佳培养转速为150～180 r/min.菌株在固态培养基中生长最适水分条件为35%,水分大于35%时蛋白酶活性较高,蛋白酶活力最高为 143.63 U/g.温度对芽孢杆菌的生长影响较为明显,在35～45℃范围内生长较好,最佳产蛋白酶温度为40℃.酸度对芽孢杆菌生长影响大,在pH5～6条件下对菌株产生不同程度抑制,菌株在酸性和偏中性条件下均能产生蛋白酶.正交试验表明,芽孢杆菌在40%含水率、35℃、pH8.0 固态培养条件下产蛋白酶活力最高.
Abstract
This study conducted tolerance tests on several strains of Bacillus stored in the laboratory and explored their enzyme production condi-tions.The results showed that the optimal pH range for Bacillus growth was6.0-8.0,the optimal growth temperature was35℃,and the optimal cultivation speed was 150-180 rpm.The optimal water condition for the growth of the strain in solid-state culture medium is 35%.When the water content isgreater than35%,the protease activity ishigher,and the highest protease activity is143.63 U/g.The temperature hasa signifi-cant impact on the growth of Bacillus,with better growth in the range of 35-45℃.The optimal temperature for protease production is 40℃.A-cidity has a significant impact on the growth of Bacillus.Under pH 5-6 conditions,the strain is inhibited to varying degrees.The strain can pro-duce protease under both acidic and neutral conditions.The orthogonal experiment showed that Bacillus had the highest protease activity under solid-state cultivation conditions of 40%water content,35℃,and pH 8.0.
关键词
芽孢杆菌/蛋白酶/生长特性/实验室检测Key words
Bacillus/Protease/Growth Characteristics/Laboratory Testing引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据