Optimization of Extraction Process for Total Flavonoids in Water Hemp Fruit by Orthogonal Experiment
This study used wild water hemp fruits from Wenchuan County,Aba Prefecture,Sichuan Province as raw materials,and used ultra-sound assisted extraction to extract total flavonoids from water hemp fruits.After single factor and orthogonal experiments,it was found that each factor had a significant impact on the extraction rate of total flavonoids.The strength of the impact from high to low was as follows:ethanol vol-ume fraction＞extraction time＞solid-liquid ratio＞extraction temperature.The optimal parameters were:extraction temperature 60 ℃,extraction time 75 minutes,ethanol volume fraction 50％,and solid-liquid ratio 1∶40.Under the optimal parameter conditions,the extraction rate of total fla-vonoids from water sesame fruit reached 2.23％.Exploring the scavenging effects of total flavonoid extract on·OH and DPPH free radicals,the scavenging rates reached 76.1％and 59.8％,respectively.This extraction process has the characteristics of simple operation,strong feasibility,and stable extraction rate.
Water Hemp FruitTotal FlavonoidsUltrasound AssistedAntioxidantOrthogonal Test
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维普
