This study used wild water hemp fruits from Wenchuan County,Aba Prefecture,Sichuan Province as raw materials,and used ultra-sound assisted extraction to extract total flavonoids from water hemp fruits.After single factor and orthogonal experiments,it was found that each factor had a significant impact on the extraction rate of total flavonoids.The strength of the impact from high to low was as follows:ethanol vol-ume fraction>extraction time>solid-liquid ratio>extraction temperature.The optimal parameters were:extraction temperature 60 ℃,extraction time 75 minutes,ethanol volume fraction 50%,and solid-liquid ratio 1∶40.Under the optimal parameter conditions,the extraction rate of total fla-vonoids from water sesame fruit reached 2.23%.Exploring the scavenging effects of total flavonoid extract on·OH and DPPH free radicals,the scavenging rates reached 76.1%and 59.8%,respectively.This extraction process has the characteristics of simple operation,strong feasibility,and stable extraction rate.
关键词
水麻果/总黄酮/超声辅助/抗氧化/正交试验
Key words
Water Hemp Fruit/Total Flavonoids/Ultrasound Assisted/Antioxidant/Orthogonal Test