云南化工2024,Vol.51Issue(11) :38-41.DOI:10.3969/j.issn.1004-275X.2024.11.09

水麻果总黄酮的超声辅助提取工艺优化及其抗氧化活性研究

Optimization of Extraction Process for Total Flavonoids in Water Hemp Fruit by Orthogonal Experiment

向君豪 罗燕丽 李心柔 梁尚雨 刘壹 任朝琴
云南化工2024,Vol.51Issue(11) :38-41.DOI:10.3969/j.issn.1004-275X.2024.11.09

水麻果总黄酮的超声辅助提取工艺优化及其抗氧化活性研究

Optimization of Extraction Process for Total Flavonoids in Water Hemp Fruit by Orthogonal Experiment

向君豪 1罗燕丽 1李心柔 1梁尚雨 1刘壹 1任朝琴1
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作者信息

  • 1. 阿坝师范学院,四川 阿坝 624000
  • 折叠

摘要

以四川省阿坝州汶川县的野生水麻果实为原料,运用超声辅助提取水麻果中的总黄酮,先后经过单因素试验和正交试验,发现各因素对总黄酮提取率都有显著影响,影响的强弱由高到低依次为:乙醇体积分数>提取时间>料液比>提取温度,最佳参数为:提取温度60 ℃、提取时间75 min、乙醇体积分数50%以及料液比1∶40.在最佳参数条件下,水麻果总黄酮的提取率达到2.23%.分别探究总黄酮提取液对.OH自由基和DPPH自由基的清除作用,清除率分别达到76.1%和59.8%.超声辅助提取工艺具有操作简单、可行性强以及提取率稳定的特点.

Abstract

This study used wild water hemp fruits from Wenchuan County,Aba Prefecture,Sichuan Province as raw materials,and used ultra-sound assisted extraction to extract total flavonoids from water hemp fruits.After single factor and orthogonal experiments,it was found that each factor had a significant impact on the extraction rate of total flavonoids.The strength of the impact from high to low was as follows:ethanol vol-ume fraction>extraction time>solid-liquid ratio>extraction temperature.The optimal parameters were:extraction temperature 60 ℃,extraction time 75 minutes,ethanol volume fraction 50%,and solid-liquid ratio 1∶40.Under the optimal parameter conditions,the extraction rate of total fla-vonoids from water sesame fruit reached 2.23%.Exploring the scavenging effects of total flavonoid extract on·OH and DPPH free radicals,the scavenging rates reached 76.1%and 59.8%,respectively.This extraction process has the characteristics of simple operation,strong feasibility,and stable extraction rate.

关键词

水麻果/总黄酮/超声辅助/抗氧化/正交试验

Key words

Water Hemp Fruit/Total Flavonoids/Ultrasound Assisted/Antioxidant/Orthogonal Test

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出版年

2024
云南化工
云南省化工研究院 云天化集团有限责任公司 云南煤化工集团有限公司 云南省化学化工学会

云南化工

影响因子:0.272
ISSN:1004-275X
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