ldentification of the Semen of Gleditsia Sinensis Lam.and G.delavayi Franch.by Ultraviolet Spectroscopy Combined with Infrared Spectroscopy
(Objective)To establish a rapid method for distinguishing the Semen of Gleditsia sinensis Lam.and G.delavayi Franch.by Ultravio-let Spectroscopy Combined with Infrared Spectroscopy.(Methods)The W-normal test method was used to identify common peaks,principal component analysis and orthogonal partial least squares discriminant analysis were used to distinguish the characteristic UV absorption spectra of the Semen of Gleditsia sinensis Lam.and G.delavayi Franch..The Fourier transform infrared spectroscopy technology was used to obtain the in-frared spectra and first-order derivative spectra of different origins of the Semen of Gleditsia sinensis Lam.and G.delavayi Franch..(Results)There are common absorption peaks in the UV spectra of the Semen of Gleditsia sinensis Lam.and G.delavayi Franch.in the methanol extrac-tion solution with different characteristics,which can be clearly identified through analysis.Compared with the Semen of Gleditsia sinensis Lam.,the average infrared spectrum of the Semen of G.delavayi Franch.has a unique vibration absorption peak near 1374 cm-1 and 1251 cm-1.(Conclusion)Both ultraviolet line group method and Fourier infrared spectroscopy can be used to distinguish the Semen of Gleditsia sinensis Lam.and G.delavayi Franch.,the methods are simple,accurate and sensitive.
万方数据
维普
