A high-performance liquid chromatography method was established to simultaneously detect the content of fumaric acid and maleic acid in fumarate fumarate fumarate fumarate.C8 chromatography column,using 0.05 mol/L potassium dihydrogen phosphate buffer methanol(95∶5,V/V)as mobile phase A and acetonitrile as mobile phase B,gradient elution.Fumaric acid has a good linear relationship within the con-centration range of 50％to 150％,with a lower limit of quantification of 0.7491 μg/mL;The linear relationship of maleic acid is good within the concentration range of LOQ-200％,with a lower limit of quantification of 0.4100 μg/mL;The RSD of all 12 test solutions in the precision test was less than 2.0％.This method has good precision and durability,and is simple and sensitive.It can be used to simultaneously detect the con-tent of fumaric acid and maleic acid in fumarate.
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