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褐煤微生物液化过程中真菌多样性演替分析

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为探究褐煤液化过程中微生物的演替规律,以一株自筛选真菌为液化微生物,利用高通量测序技术考察不同pH、液化时间、煤炭添加对液化过程中微生物菌群的影响.结果发现,不同培养基pH对煤炭液化过程中的物种组成有明显影响,pH=4和pH=8的独有物种分别有2种和3种,其中g_Trichoderma主要来自pH=4的样本,占90.9%.导致不同pH样本组间物种组成极度差异的物种有Hannaella coprosmae和Trichoderma等,其P值均<0.0005.Wallemia和Archaeorhizomyces是造成不同液化天数显著差异物种.在pH、液化时间、加煤情况3个环境因子中,既具有强相关性且显著的物质是Hannaella coprosmae及Papiliotrema.
Analysis of Fungal Diversity Succession During Lignite Microbial Liquefaction Process
In order to explore the succession of microorganisms in the liquefaction of lignite,a self screened fungus was selected as the liquefac-tion microorganism,and high-throughput sequencing technology was used to investigate the effects of different pH,liquefaction time,and coal addition on the microbial community during the liquefaction process.As a result,it was found that different pH values of the culture medium had a significant impact on the species composition during coal liquefaction.There were 2 and 3 unique species at pH 4 and pH 8,respectively.Among them,g_Trichoderma mainly came from samples at pH 4,accounting for 90.9%.The species that cause extreme differences in species composition between different pH sample groups include Hannaella cuprosae and Trichoderma,with P<0.0005 for both.Wallemia and Archae-olhizomyces are species that cause significant differences in liquefaction days.Among the three environmental factors of pH,liquefaction time,and coal addition,the substances with strong correlation and significance are Hannaella cuprosae and Papiliotrema.

LigniteHigh-throughput SequencingBio-diversityBiological Liquefaction

蒋翥、周静、张金晶、石开仪

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黔南民族师范学院 化学化工学院,贵州 都匀 558000

褐煤 高通量测序 生物多样性 生物液化

2024

云南化工
云南省化工研究院 云天化集团有限责任公司 云南煤化工集团有限公司 云南省化学化工学会

云南化工

影响因子:0.272
ISSN:1004-275X
年,卷(期):2024.51(12)