Analysis of Fungal Diversity Succession During Lignite Microbial Liquefaction Process
In order to explore the succession of microorganisms in the liquefaction of lignite,a self screened fungus was selected as the liquefac-tion microorganism,and high-throughput sequencing technology was used to investigate the effects of different pH,liquefaction time,and coal addition on the microbial community during the liquefaction process.As a result,it was found that different pH values of the culture medium had a significant impact on the species composition during coal liquefaction.There were 2 and 3 unique species at pH 4 and pH 8,respectively.Among them,g_Trichoderma mainly came from samples at pH 4,accounting for 90.9%.The species that cause extreme differences in species composition between different pH sample groups include Hannaella cuprosae and Trichoderma,with P<0.0005 for both.Wallemia and Archae-olhizomyces are species that cause significant differences in liquefaction days.Among the three environmental factors of pH,liquefaction time,and coal addition,the substances with strong correlation and significance are Hannaella cuprosae and Papiliotrema.