首页|鸡冠刺桐枝叶黄酮提取工艺及抑菌效果研究

鸡冠刺桐枝叶黄酮提取工艺及抑菌效果研究

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以鸡冠刺桐枝叶为研究材料,通过单因素和正交试验研究了其黄酮类化合物的最佳提取条件.结果表明,最佳提取条件为:以50%的乙醇溶液作为提取剂,按照1∶50(g/mL)的料液比在80℃的水浴温度下恒温提取60min,随后用超声波处理40 min,总黄酮的提取率为25.4 mg/g.以最优提取工艺提取的溶液为原料,通过纸片扩散法对大肠杆菌和金黄色葡萄球菌进行了抑菌活性研究,结果显示,优化后的提取液对金黄色葡萄球菌表现出一定的抑菌效果,对大肠杆菌没有明显的抑菌效果.
Extraction of Flavonoids from Leaves and Branches of Erythrina Crista-galli and Their Antibacterial Effect
Leaves and branches of Erythrina crista-galli served as the basis for this study.Through single factor and orthogonal testing,we de-termined the best conditions for extracting flavonoids.The ideal extraction process involved using a 50%ethanol solution as the solvent,with a material-to-solvent ratio of 1∶50.Extraction was conducted at a constant temperature of 80℃ in a water bath for 60 minutes,followed by an additional 40 minutes of ultrasonic treatment.This yielded a total flavonoid extraction of 25.4 mg/g.Utilizing this optimized extract,we ex-plored its antibacterial properties against Escherichia coli and Staphylococcus aureus using the paper diffusion method.The results demonstrated that the extract displayed antibacterial properties against Staphylococcus aureus,while it did not have a considerable effect on Escherichia coli.

Erythrina Crista-galliFlavonoidsExtraction TechnologyBacteriostasis

黄涛、罗慧、董亭亭、尹淼、任妤佶、向巧、龚安界

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四川文理学院化学化工学院,四川 达州 635000

特色植物开发研究四川省高校重点实验室,四川 达州 635000

鸡冠刺桐 黄酮类化合物 提取工艺 抑菌

2024

云南化工
云南省化工研究院 云天化集团有限责任公司 云南煤化工集团有限公司 云南省化学化工学会

云南化工

影响因子:0.272
ISSN:1004-275X
年,卷(期):2024.51(12)