Leaves and branches of Erythrina crista-galli served as the basis for this study.Through single factor and orthogonal testing,we de-termined the best conditions for extracting flavonoids.The ideal extraction process involved using a 50％ethanol solution as the solvent,with a material-to-solvent ratio of 1∶50.Extraction was conducted at a constant temperature of 80℃ in a water bath for 60 minutes,followed by an additional 40 minutes of ultrasonic treatment.This yielded a total flavonoid extraction of 25.4 mg/g.Utilizing this optimized extract,we ex-plored its antibacterial properties against Escherichia coli and Staphylococcus aureus using the paper diffusion method.The results demonstrated that the extract displayed antibacterial properties against Staphylococcus aureus,while it did not have a considerable effect on Escherichia coli.
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