(Objective)A nanozymatic colorimetric method for the determination of nitrite(NO-2)in tobacco leaf samples was established.(Meth-ods)Based on the excellent peroxidase-like(POD)catalytic activity of copper and iodine doped carbon dots(Cu,I-CDs),colorless 3,3',5,5'-tetramethylbenzidine(TMB)can be oxidized to blue oxTMB under acidic conditions and in the presence of H2O2,and an obvious absorption peak appears at 652 nm.With the addition of nitrite(NO2-),based on the formation of NO2,the peroxidase-like catalytic activity of the system was enhanced,and the blue color of the solution was deepened.A new colorimetric method for the detection of nitrite was established.(Results)In the range of 0.05-17.5 mg/L,the A654 value showed a good linear relationship with the concentration of NO2-,and the detection limit was 0.0126 mg/L(S/N=3).(Conclusion)At the same time,the constructed detection system has outstanding performance in recovery rate and an-ti-interference,and has good detection accuracy,and the recovery rate of standard addition is above 96.9％.The sensor has been successfully applied to the detection of tobacco leaf samples.The method has the characteristics of fast,simple,stable and strong specificity.
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