Detection of Bactericidal Sodium Benzoate in Oral Liquid Preparations and the Addition Analysis
(Objective)An analysis method was developed to determine sodium benzoate in oral liquid prepaertions,and the detection status of multiple batches of samples was also analyzed.(Methods)The HPLC-DAD method was adopted and separated by an Agilent ZORAX Eclipse XDB C18(250 mm × ø4.6 mm,5 μm)column with Methanol-0.02 mol/L sodium dihydrogen phosphate solution(adjusted pH to 3.7 with phos-phates)(30∶70,V/V)as the mobile phase.The column temperature was 30 ℃,the detection wavelength was 228 nm And injection quantity was 10 μL.(Results)The peak area and the concentration of sodium benzoate in 0.0131-0.261 mg·mL-1(r=1.0000);the limit of detection is 0.105 μg·mL-1 and the limit of quantification is 0.209 μ g·mL-1.The average recovery of complex syrup and mixture was 98.3％and 101.6％,and the RSD was 0.82％(n=9)and 0.76％(n=9),respectively;for 105 batches of 56 varieties,the content of sodium benzoate de-tected varied greatly.(Conclusion)The method is simple,rapid,specific and sensitive.It can be used for the quantitative screening of sodium benzoate in oral liquid preparations.
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