Quality Control Research on Yinju Mixture Based on Fingerprint Spectrum Combined with Chemometrics Analysis and Multi-component Content Determination
To establish the HPLC fingerprint of Yinju mixture and determine the contents of active components(chl orogenic acid,luteolin and glycyrrhizin)in different batches,so as to provide a reference for the quality control of Yinju mixture.Agilent C18 column was utilized,apply-ing a gradient elution with a mobile phase of acetonitrile and a 0.1％phosphoric acid solution;the flow rate was set at 1 mL/min;the column temperature was 30 ℃;the detection wavelength was 327 nm;and the injection volume was 10 μL.The HPLC fingerprint was established for 10 batches of Yinju Mixture,with similarity evaluation and chemometric analysis conducted,alongside the determination of the contents of chloro-genic acid,luteolin,and glycyrrhizic acid.A total of 11 common peaks were identified,all with similarities greater than 0.95,and the contents of three components were quantified:chlorogenic acid ranging from 825.68 to 850.17 μg/mL,glycyrrhizic acid from 17.31 to 24.02 µg/mL,and luteolin from 34.31 to 34.98 μg/mL.Cluster analysis and principal component analysis categorized the 10 batches of Yinju Mixture into three general groups.The variable importance in the projection(VIP)values for peak 1,peak 2(chlorogenic acid),and peak 4 all exceeded 1,indicating a significant influence on the respective principal components,particularly peak 1 on the first,peak 2 on the second,and peak 3 on the third.An orthogonal partial least squares-discriminant analysis(OPLS-DA)model was constructed using the common peak areas as varia-bles,with RX2 at 0.727 and Q2 at 0.5 1,both exceeding 0.5,indicating that the model has good stability and reliability.The identified stand-ards of chlorogenic acid,glycyrrhizic acid,and luteolin are potential quality markers.The fingerprint and multi-component content determina-tion methods established in this study are stable and reliable,simple and fast,and can be used for the quality control of Yinju Mixture.
Yinju MixtureFingerprintsStoichiometryContent DeterminationQuality MarkerQuality Control
万方数据
维普
