Study on extraction and hypoglycemic activity of protein from Gynura medica
In order to develop the hypoglycemic medicinal plant named Gynura medica,the extraction process of the active protein of Gynura medica was studied in terms of extraction solvent,heating method,extraction time,extrac-tion temperature and material liquid ratio,and the effects of the extracts obtained by different processes on α-Am-ylase and α-glucosidase was detected.The extraction conditions were optimized by response surface methodology.The results showed that the maximum extraction rate of protein was 10.8%under the following conditions:extrac-tion temperature 62℃,extraction time 3 h,material liquid ratio 1∶30,extraction solvent PBS.The extraction condi-tions of the extractor with the highest inhibition rate of α-amylase were as follows:extraction temperature 59℃,extraction time 3h,material liquid ratio 1∶30,extraction solvent PBS,the maximum inhibition rate was 31%;The extraction conditions of the extractor with the highest inhibition rate of α-glucosidase were as follows:extraction temperature 52℃,extraction time 5 h,material liquid ratio 1∶30,extraction solvent PBS.The maximum inhibition rate of α-glucosidase was 13.60%.The inhibition rate of α-amylase increased with the increase of protein con-tent.Considering the protein content and purity of the extract,it can be inferred that the extractor of Gynura medica contained a high α-amylase inhibition activity protein.
万方数据
维普
