Evaluation of the alleviating effect of Nitraria Tangutorum Bobr.leaves extract on ulcerative colitis induced by DSS in mice and its antioxidant stress effect based on HepG-2 cells
The study aims to explore the alleviating effect of Nitraria Tangutorum Bobr.leaves on mice with DSS in-duced ulcerative colitis and its possible mechanism.Mice with ulcerative colitis induced by DSS were treated with the extract of Nitraria Tangutorum Bobr.leaves,the histopathological changes of colon were observed by HE staining and disease activity index(DAI),and enzyme-linked immunosorbent assay(ELISA)was used to detect the content of inflammatory factors IL-6、IL-1β、TNF-α.In addition,HPLC-ESI-HRMS/MS was used to identify the chemical components of Nitraria Tangutorum Bobr.Leaves.On the basis of the chemical components,the levels of antioxidant enzymes such as reactive oxygen species(ROS),glutathione transferase(GSH),superoxide dismutase(SOD)and catalase(CAT)were detected based on HepG-2 cells.Compared with normal group,colon DAI score and serum levels of IL-6,IL-1β and TNF-α were increased in the model group(P＜0.01).ROS levels were increased in the model group,while GSH,SOD and CAT were decreased in the HepG-2 cells(P＜0.05).Compared with the model group,DAI,IL-6,IL-1β and TNF-α levels in serum were decreased(P＜0.01),symptoms of ulcerative colitis were alleviated,ROS levels in HepG-2 cells were decreased,and GSH,SOD and CAT levels were increased in Nitraria Tangutorum Bobr.leaves group(P＜0.05).Conclusion Nitraria Tangutorum Bobr.leaves have therapeutic effects on DSS induced ulcerative colitis,and the mechanism of action may be closely related to reducing the levels of in-flammatory factors and active oxygen,increasing the levels of antioxidant enzymes at the same time.
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