Bioinformatics study and immune analysis on the mechanism of Sangju decoction in the treatment of respiratory virus infection
This study aims to explore the mechanism of Sangju decoction in the treatment of respiratory tract virus infection from the molecular level by means of bioinformatics,network pharmacology,immune infiltration analysis and molecular docking.The chemical constituents and targets of Sangju decoction were obtained by TCMSP data-base.The upper respiratory tract infection related chips were searched and downloaded through the geo database,and the differential mRNA was extracted.After sorting out the matrix,the immune infiltration analysis was performed using the R language cibersortx package.The key differential mRNA obtained by limma package analysis is used as the disease target,and the therapeutic target is obtained after mapping with the drug target.The string 11.5 database was used for PPI analysis,and the R language clusterprofiler package was used for go and KEGG enrichment analy-sis(p＜0.05 and Qvalue＜0.2).The relevant network diagram was built by using Cytoscape 3.8.1 software and the core targets by cytoHubba was screened;AutoDockVina software was used for molecular docking verification.A total of 169 active components and 248 action targets were obtained from 8 drugs in Sangju decoction.CIBERSORTx was used to analyze 22 kinds of immune cells.PPI analysis showed that CXCL8,PPARy,IL1β and MAPK14 were the key targets.GO analysis and enrichment of KEGG pathway suggested that the mechanism of Sangjudecoction was mainly involved in biological processes such as lipopolysaccharide response,macrophage-derived foam cell differen-tiation,leukocyte chemotaxis and activation of MAPK activity,as well as AGE-RAGE,Toll-like receptors,EBV in-fection,IL-17,HIF-1,influenza An and other signal pathways.The results of molecular docking showed that the main active components in the prescription had stable binding activity with key targets.Sangju decoction may play a role in improving cellular immune state,inflammatory reaction and anti-virus infection by acting on IL1β,MAPK14 and other active components such as naringin,luteolin,quercetin and arachidonic acid.It is a multi-component,multi-protein target and multi-pathway process.
万方数据
维普
