Effects and Mechanism of Cuscuta chinensis Polysaccharides on Improving Motor Ability in Rats Based on AMPK/PGC-1α Signaling Pathway
[Purpose]To study the effects and potential mechanism of Cuscuta chinensis polysac-charides(CCPs)on improving motor ability in rats,based on adenosine monophosphate activated pro-tein kinase(AMPK)/peroxisome proliferator activator receptor γ coactivator 1α(PGC-1α)signaling pathway.[Methods]Fifty rats were divided into normal control(NC)group,exercise model(EM)group and CCPs low-dose(CCPs-L),medium-dose(CCPs-M)and high-dose(CCPs-H)groups(10,200 and 400 mg/kg).Except NC group,all rats in the other groups needed swimming training.After swimming to exhaustion,the anti-fatigue indexes in serum,the indexes of energy metabolism and oxidative stress in liver tissue,the mRNA expression levels of AMPKα and PGC-1α,and the protein expression levels of phosphorylated AMPKα(p-AMPKα),AMPKα and PGC-1α were measured.[Results]Compared with EM group,swimming exhaustion time of CCPs groups was significantly or extremely significantly prolonged;the contents of blood urea nitrogen,lactic acid and corticoster-one in serum and malondialdehyde in liver tissue were extremely significantly decreased;the contents of serum testosterone and liver glycogen,and the activities of Na+-K+-ATPase,Ca2+-Mg2+-ATPase,superoxide dismutase and glutathion peroxidase in liver tissue were significantly increased;the mRNA and protein levels of AMPKα in liver tissue had no significant changes,but the mRNA level of PGC-1α and protein levels of p-AMPKα and PGC-1α were extremely significantly increased.[Conclusion]CCPs can improve the exercise ability of rats trained with high intensity,which is related to anti-fatigue,increasing energy reserves,inhibiting oxidative stress and activating AMPK/PGC-1 α signaling pathway.
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