Metabolomics Analysis of the Development Process of Camellia oleifera Seed Kernel
[Purpose]To understand the changes of kernel metabolites during the development of Camellia oleifera fruits.[Methods]Using different developmental stages of C.oleifera kernels as materials,combined with liquid chromatography mass spectrometry(LC-MS/MS)metabolomics tech-niques,the metabolic changes of C.oleiferakernels during development were analyzed.[Results]A total of 1006 differential metabolites were identified,belonging 15 categories,mainly including lip-ids and lipid-like molecules,phenylpropane and polyketones,organic acids and their derivatives,ben-zenoids,organic oxygen compounds,organoheterocyclic compounds,nucleosides,nucleotides and analogs.The annotated main metabolic pathways included metabolic pathways,biosynthesis of sec-ondary metabolites,biosynthesis of plant secondary metabolites,biosynthesis of amino acids,and metabolism of 2-oxalic acid.There were significant differences in metabolites in the kernels of C.oleifera at different developmental stages.F1 and F2 periods had the least metabolites,only inclu-ding 3-methyl-1-(2,4,6-trihydroxyphenyl)-1-butanone,L-glutathione,and sucrose,indicating slow growth of the kernels in the early stages.In F3 period,there were the largest number of kernel meta-bolites,especially the relative contents of phenylpropane and polyketones,benzenoids,lipids and lip-id molecules,organic acids and their derivatives,and organic heterocyclic compounds changed obvi-ously;the relative contents of melanin,tyrosine,phenylalanine,L-glutathione,1-methylnenenebc guanine,3,4-dihydro-2H-1-benzopyran-2-one,(S)-pinocenbrin,and kaempferol-3-O-rutoside were re-latively high,indicating that it played an important role in the formation of kernels during the rapid expansion period of fruits.The metabolites in F4 and F5 periods gradually decreased,and the kernel entered the oil accumulation period.[Conclusion]This study uses metabolomics methods to under-stand the changes in kernel metabolites during the development process of C.oleifera fruits,which can provide a theoretical basis for high-quality and high-yield cultivation and timely harvesting of C.oleiferafruits.
Camellia oleiferadevelopment process of fruitseed kernelmetabolome
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