土壤熏蒸对三七连作土壤微生物群落的影响
Effects of Soil Fumigation on Microbial Community under Continuous Cropping of Panax notoginseng
王豪吉 1陈鹏崟 1王勇 2魏富刚 3杨绍周 3官会林 1徐武美1
作者信息
- 1. 云南师范大学 高原特色中药材种植土壤质量演变退化与修复云南省野外科学观测研究站,云南 昆明 650500
- 2. 文山学院 文山三七研究院,云南 文山 663099
- 3. 文山苗乡三七科技有限公司,云南 文山 663099
- 折叠
摘要
用氯化苦熏蒸三七连作土壤,分析土壤微生物群落,并与林下健康三七种植土壤相比较,探究氯化苦熏蒸消减三七连作障碍的微生物学机理.结果表明:三七种植 18 个月后,试验组三七存活率为63.0%,而对照组三七存活率仅为 2.0%.高通量测序与生信分析表明:与林下健康三七种植土壤相比,田间三七连作导致土壤微生物多样性降低(P<0.05),镰刀菌属(Fusarium)、癣囊腔菌属(Plectosphaerella)和链格孢属(Alternaria)等潜在病原菌群的相对丰度升高(P<0.05),伯克霍尔德菌属(Burkholderia)和慢生根瘤菌属(Bradyrhizobium)等潜在有益菌群的相对丰度降低(P<0.05).氯化苦熏蒸可有效降低三七连作土壤中病原菌群的相对丰度(P<0.05),调节微生物群落,显著提高连作三七存活率,是消减三七连作障碍,促进三七可持续栽培的有效措施.
Abstract
To investigate the microbiological mechanism of chloropicrin fumigation(CF)in reducing continuous cropping obstacles of Panax notoginseng,the soil microbial communities under CP were analyzed and compared with the healthy soil cultivating P.notoginseng under forests.The results showed that after 18 months of cultivation,the survival rate of P.notoginseng in the experimental group was 63.0%,while the survival rate of P.notoginseng in the control group was only 2.0%.High throughput sequencing and bioinformatic analyses showed that compared with the soil under forest,the continuous cropping of P.notoginseng led to the decrease of soil microbial diversity(P<0.05),moreover,the relative abundance of pathogenic microorganisms such as Fusarium,Plectosphaerella,and Alternaria was increased,and the beneficial Burkholderia and Bradyrhizobium was decreased(P<0.05).CF reduced the relative abundance of pathogens in soil(P<0.05),regulated microbial community,and improved the survival rate of P.notoginseng under continuous cropping,which is an effective measure to reduce continuous cropping obstacle and promote sustainable cultivation of P.no-toginseng.
关键词
三七/氯化苦/熏蒸/微生物群落/连作障碍Key words
Panax notoginseng/Chloropicrin/Fumigation/Microbial community/Continuous cropping obstacle引用本文复制引用
基金项目
国家自然科学基金(31901126)
云南省兴滇英才支持计划(XDYC-QNRC-2022-0247)
云南省院士专家工作站项目(202305AF150027)
云南省院士专家工作站项目(YSZJGZZ-2021062)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据