Effects of Soil Fumigation on Microbial Community under Continuous Cropping of Panax notoginseng
To investigate the microbiological mechanism of chloropicrin fumigation(CF)in reducing continuous cropping obstacles of Panax notoginseng,the soil microbial communities under CP were analyzed and compared with the healthy soil cultivating P.notoginseng under forests.The results showed that after 18 months of cultivation,the survival rate of P.notoginseng in the experimental group was 63.0%,while the survival rate of P.notoginseng in the control group was only 2.0%.High throughput sequencing and bioinformatic analyses showed that compared with the soil under forest,the continuous cropping of P.notoginseng led to the decrease of soil microbial diversity(P<0.05),moreover,the relative abundance of pathogenic microorganisms such as Fusarium,Plectosphaerella,and Alternaria was increased,and the beneficial Burkholderia and Bradyrhizobium was decreased(P<0.05).CF reduced the relative abundance of pathogens in soil(P<0.05),regulated microbial community,and improved the survival rate of P.notoginseng under continuous cropping,which is an effective measure to reduce continuous cropping obstacle and promote sustainable cultivation of P.no-toginseng.