首页|转录组分析初步揭示脂质代谢在增强马铃薯S.commersonii耐冻中的作用

转录组分析初步揭示脂质代谢在增强马铃薯S.commersonii耐冻中的作用

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马铃薯(Solanum tuberosum L.)是全球最重要的块茎类粮食作物.全球极端气候的频发及马铃薯种植区域的扩大,使得马铃薯易受极端气候灾害的影响,其中冻害是马铃薯生产中常见的气候灾害之一.栽培马铃薯不耐霜冻,冻害一旦发生严重影响马铃薯的生产.通过对低温敏感和低温耐受的两种不同马铃薯材料进行低温处理,转录组测序,检测到的22 717个基因中有7 522个差异表达基因(DEGs),KEGG富集分析表明脂质代谢的DEGs被特异性富集,进一步分析发现磷酸乙醇胺胞苷转移酶1(PECT1)、过氧化物酶体3-酮酰辅酶A硫酰酶3(PKT3)、甾醇甲基转移酶2(SMT2)、甾醇甲基转移酶3(SMT3)和长链酰基-CoA合成酶8(LACS8)等5个脂质代谢途径中关键酶的编码基因在低温处理后表达量上调,与抗寒性高度相关.
Transcriptome Analysis of Wild Potato Species S.commersonii under Low Temperature
Potato(Solanum tuberosum L.)is the most important tuber crop globally.The increasing fre-quency of extreme global climates and the expansion of potato cultivation areas make potatoes vulner-able to extreme weather disasters.Frost damage is a common climate disaster in potato production as cultivated potatoes are not frost-resistant.Once severe frost damage occurs,it significantly affects po-tato production.Through transcriptome sequencing of two different potato materials with cold sensi-tivity and cold tolerance,and found that there were 7 522 differential genes among 22 717 detected genes.KEGG analysis revealed that cold treatment showed specific enrichment of lipid metabolism at the transcriptional level.After further analysis five candidate genes of the lipid metabolism pathway,PKT3,SMT2,SMT3,PECT1 and SMT3,were up-regulated after low-temperature treatment and highly correlated with cold resistance.

PotatoFreezingTranscriptomeLipid metabolism

郑昊吉、王玺尧、吴玺、袁力、赵强彪、高建莉、郭磊、祝光涛

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云南师范大学生命科学学院,马铃薯科学研究院,云南省马铃薯生物学重点实验室,云南昆明,650500

中国农业科学院(深圳)农业基因组研究所,广东深圳,518000

文山州农业科学院,云南文山,663000

马铃薯 抗寒性 转录组 脂质代谢

广东省基础与应用基础研究重大项目

2021B0301030004

2024

云南师范大学学报(自然科学版)
云南师范大学

云南师范大学学报(自然科学版)

CSTPCD
影响因子:0.54
ISSN:1007-9793
年,卷(期):2024.44(3)
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