Optimization of Genetic Transformation Efficiency of Cultivated and Wild Potato
In order to optimize the genetic transformation system of cultivated and wild potato and im-prove the transformation efficiency,cultivated potato Desiree(De)and wild potato Solanum commer-sonii(Sc)were selected as receptor materials,and the infection time was set.De and Sc were infected at 10,20,40,60 min and 80 min,respectively.The results showed that the conversion efficiency reached 50.86％when the infection time of De was 40 min,and the conversion efficiency reached 96.3％when the infection time of Sc was 20 min.The results of this study provided the best infection duration of cultivated species De and wild species Sc,greatly improved the conversion efficiency of these two re-ceptors,optimized their genetic transformation system,and saved costs,which is of great significance for potato genetic engineering breeding.
万方数据
维普
