云南师范大学学报(自然科学版)2024,Vol.44Issue(3) :40-44.DOI:10.7699/j.ynnu.ns-2024-035

栽培和野生马铃薯遗传转化效率优化

Optimization of Genetic Transformation Efficiency of Cultivated and Wild Potato

罗永超 康月 吴秋菊 王静蕾 刘香 思彤 祝光涛
云南师范大学学报(自然科学版)2024,Vol.44Issue(3) :40-44.DOI:10.7699/j.ynnu.ns-2024-035

栽培和野生马铃薯遗传转化效率优化

Optimization of Genetic Transformation Efficiency of Cultivated and Wild Potato

罗永超 1康月 1吴秋菊 1王静蕾 1刘香 1思彤 1祝光涛1
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作者信息

  • 1. 云南师范大学生命科学学院,马铃薯科学研究院,云南省马铃薯生物学重点试验室,云南昆明 650500
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摘要

为优化栽培和野生马铃薯遗传转化体系,提高转化效率,选择栽培马铃薯Desiree(De)和野生马铃薯Solanum commersonii(Sc)作为受体材料,设5个侵染时间梯度:10、20、40、60 min和80 min,分别对De和Sc进行侵染.结果表明,De的侵染时间为40 min时,转化效率最高,达50.86%,Sc的侵染时间为20 min时,转化效率最高,达96.3%.该研究结果提供了栽培种De和野生种Sc的最佳侵染时间,极大地提高了这两份受体材料的转化效率,优化其遗传转化体系,节约成本,对马铃薯基因工程育种具有重大意义.

Abstract

In order to optimize the genetic transformation system of cultivated and wild potato and im-prove the transformation efficiency,cultivated potato Desiree(De)and wild potato Solanum commer-sonii(Sc)were selected as receptor materials,and the infection time was set.De and Sc were infected at 10,20,40,60 min and 80 min,respectively.The results showed that the conversion efficiency reached 50.86%when the infection time of De was 40 min,and the conversion efficiency reached 96.3%when the infection time of Sc was 20 min.The results of this study provided the best infection duration of cultivated species De and wild species Sc,greatly improved the conversion efficiency of these two re-ceptors,optimized their genetic transformation system,and saved costs,which is of great significance for potato genetic engineering breeding.

关键词

马铃薯/遗传转化/转化效率

Key words

Potatoes/Genetic transformation/Conversion efficiency

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基金项目

云南省科技厅基础研究项目(202001AV070003)

出版年

2024
云南师范大学学报(自然科学版)
云南师范大学

云南师范大学学报(自然科学版)

CSTPCD
影响因子:0.54
ISSN:1007-9793
参考文献量12
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