The Quality Identification of Astragalus membranaceus from Different Years by Root Morphology and Flavonoids
Studying on the accumulation of total flavonoids and Calycosin-7-Glucoside in root of A.membranaceus root in 6 different years of 3 to 8,and comparing the morphology of the root to provide a basis for standardized cultivation and rational harvesting of it.The anatomical structure and the main position of flavonoids were observed by microscopic section staining.Measuring the biggest diameter of different samples by vernier calliper,and to measure total flavonoids and Calycosin-7-Glucoside in root of A.membranaceus from 6 different years by utraviolet visible spectrophotometry and high perform-ance liquid chromatography(HPLC).The results showed that the average diameter of the 6 different years was significantly different among one another.The phloem is the main storage site of flavonoids in A.membranaceus roots,and the flavonoids of 5 years root phloem accounts for the highest propor-tion,and the total flavonoids and Calycosin-7-Glucoside of the 5 years was the highest.According to the above results,it was advisable that the reasonable harvest of A.membranaceus should be focused on the 5 years.