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重金属铜对土壤磷酸酶活性的影响及其动力学特征

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为了获得不同浓度的Cu对土壤磷酸酶活性的影响及其动力学特征,研究了 Cu与土壤磷酸酶活性和酶促反应参数之间的关系.结果表明:土壤磷酸酶活性随着底物浓度的增加而增加;在相同底物浓度下,土壤磷酸酶活性随Cu浓度的增加而降低;底物浓度、铜浓度相同时磷酸酶活性存在差异,这与土壤磷酸酶的酸度和碱度有关;铜与土壤磷酸酶的最大反应速度Vmax呈现极显著相关,表明可以用Vmax来表征土壤受重金属Cu的污染水平.
Effects of Copper on Soil Phosphatase Activity and Its Kinetic Characteristics
In order to obtain the effects of different concentrations of copper on soil phosphatase activity and its kinetic characteristics,the relationship between copper and soil phosphatase activity and enzy-matic reaction parameters was studied.The results showed that soil phosphatase activity increased with the increase of substrate concentration.At the same substrate concentration,soil phosphatase ac-tivity decreased with the increase of copper concentration.When the substrate concentration and cop-per concentration are the same,there are differences in phosphatase activity,which is related to the a-cidity and alkalinity of soil phosphatase.There is a very significant correlation between copper and the maximum reaction rate Vmax of soil phosphatase,indicating that Vmax can be used to characterize the soil pollution level with heavy metal Cu.

Heavy metalCopperSoil phosphataseKinetic parametersSoil pollution

范梦林、樊芳玲、石炳春、唐振亚、姚昊翊

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云南师范大学能源与环境科学学院,云南 昆明 650500

云南省农村能源工程重点实验室,云南 昆明 650500

昆明理工大学 现代农业工程学院,云南 昆明 650500

重金属 土壤磷酸酶 酶动力学参数 土壤污染

2024

云南师范大学学报(自然科学版)
云南师范大学

云南师范大学学报(自然科学版)

CSTPCD
影响因子:0.54
ISSN:1007-9793
年,卷(期):2024.44(6)