Kitchen wastewater contains high organic matter,and high ammonia nitrogen,so adoption of computer-aided bacteria selection technology can separate and obtain good aerobic denitrifying bacterial strain.Test results are:when the strain culture temperature is 35 ℃,with the conditions of the intermittent oscillation and strain culture time is 108 h,the amount of live aerobic denitrifying bacterial strain in culture medium reaches 8.83×107 cfu/mL.When the home-made sludge contained with microbial inoculum was added into the SBR reactor,the ammonia nitrogen removal rate in the outlet water of the reactor reached 82.10%~87.92%,compared with the ammonia nitrogen removal rate without microbial inoculum,the removal rate increased by 19.5%,it shows the aerobic denitrifying microbial inoculum has strengthening effect to denitrogenation from wastewater.