Overexpression of WRKY25 Causes Early Flowering in Arabidopsis under Long-day Conditions
The WRKY gene superfamily is one of the largest transcriptional regulators which mainly exists in plants. Arabidopsis WRKY25 is one of the group Ⅰ WRKY proteins and responds to both biotic and abiotic stresses. GUS staining and qRT-PCR analysis show that the expression of WRKY25 mainly focuses on roots, rosette leaves and cau-line leaves. Transgenic plants over-expressing WRKY25 show early flowering under long-day conditions when compared with wild-type plants. RT-PCR analysis demonstrates that the expression of API is up-regulated in 21 d-old and 27 d-old transgenic plants. Thus, it is possible that WRKY25 affects flowering through up-regulation of API directly or indirectly.
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