首页|Plant phylogenomics based on genome-partitioning strategies:Progress and prospects

Plant phylogenomics based on genome-partitioning strategies:Progress and prospects

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The rapid expansion of next-generation sequencing (NGS) has generated a powerful array of approaches to address fundamental questions in biology. Several genome-partitioning strategies to sequence selected subsets of the genome have emerged in the fields of phylogenomics and evolutionary genomics. In this review, we summarize the applications, advantages and limitations of four NGS-based genome-partitioning approaches in plant phylogenomics: genome skimming, transcriptome sequencing (RNA-seq), restriction site associated DNA sequencing (RAD-Seq), and targeted capture (Hyb-seq). Of these four genome-partitioning approaches, targeted capture (especially Hyb-seq) shows the greatest promise for plant phylogenetics over the next few years. This review will aid researchers in their selection of appropriate genome-partitioning approaches to address questions of evolutionary scale, where we anticipate continued development and expansion of whole-genome sequencing strategies in the fields of plant phylogenomics and evolutionary biology research.

Plant phylogenomicsNext-generation sequencingWhole-genome sequencingGenome skimmingRAD-SeqTargeted capture

Xiangqin Yu、Dan Yang、Cen Guo、Lianming Gao

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Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China

Kunming Col ege of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan, 650201, China

Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, 650201, China

This study was supported by the Large-scale Scientific Facilities of the Chinese Academy of SciencesStrategic Priority Research Program of the Chinese Academy of SciencesProgram of Science and Technology Tal-ents Training of Yunnan Province

2017-LSFGBOWS-01XDB310000002017HA014

2018

植物多样性(英文)
中国科学院昆明植物研究所,中国植物学会

植物多样性(英文)

CSTPCDCSCDSCI
影响因子:0.617
ISSN:2096-2703
年,卷(期):2018.40(4)
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