BrrTCP4b interacts with BrrTTG1 to suppress the development of trichomes in Brassica rapa var.rapa

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外文摘要：The number of trichomes significantly increased in CRISPR/Cas9-edited BrrTCP4b turnip(Brassica rapa var.rapa)plants.However,the underlying molecular mechanism remains to be uncovered.In this study,we performed the Y2H screen using BrrTCP4b as the bait,which unveiled an interaction between BrrTCP4b and BrrTTG1,a pivotal WD40-repeat protein transcription factor in the MYB-bHLH-WD40(MBW)complex.This physical interaction was further validated through bimolecular luciferase complementa-tion and co-immunoprecipitation.Furthermore,it was found that the interaction between BrrTCP4b and BrrTTG1 could inhibit the activity of MBW complex,resulting in decreased expression of BrrGL2,a positive regulator of trichomes development.In contrast,AtTCP4 is known to regulate trichomes development by interacting with AtGL3 in Arabidopsis thaliana.Overall,this study revealed that BrrTCP4b is involved in trichome development by interacting with BrrTTG1 in turnip,indicating a divergence from the mechanisms observed in model plant A.thaliana.The findings contribute to our understanding of the regulatory mechanisms governing trichome development in the non-model plants turnip.

外文关键词：

TCP transcription factorMBW complexTrichome developmentTurnip

作者：

Cheng Li、Li Zhang、Hefan Li、Yuanwen Duan、Xuemei Wen、Yongping Yang、Xudong Sun

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作者单位：

The Germplasm Bank of Wild Species,Yunnan Key Laboratory of Crop Wild Relatives Omics,Institute of Tibetan Plateau Research at Kunming,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China

University of Chinese Academy of Sciences,Beijing,China

Tibet Plateau Institute of Biology,Lhasa 850001,Tibet,China

基金：

Strategic Priority Research Program of the Chinese Academy of Sciences,Pan-Third Pole Environment Study for a Green Silk RoaSecond Tibetan Plateau Scientific Expedition and Research(STEP)programScience and Technology Program of Xizang Autonomous Region

项目编号：

XDA20040103062019QZKK0502XZ202001ZY0003G

出版年：

2024

DOI：

10.1016/j.pld.2024.03.003

植物多样性(英文)

中国科学院昆明植物研究所，中国植物学会

植物多样性(英文)

CSTPCD

影响因子：0.617

ISSN：2096-2703

年,卷(期)：2024.46(3)