Abstract
The number of trichomes significantly increased in CRISPR/Cas9-edited BrrTCP4b turnip(Brassica rapa var.rapa)plants.However,the underlying molecular mechanism remains to be uncovered.In this study,we performed the Y2H screen using BrrTCP4b as the bait,which unveiled an interaction between BrrTCP4b and BrrTTG1,a pivotal WD40-repeat protein transcription factor in the MYB-bHLH-WD40(MBW)complex.This physical interaction was further validated through bimolecular luciferase complementa-tion and co-immunoprecipitation.Furthermore,it was found that the interaction between BrrTCP4b and BrrTTG1 could inhibit the activity of MBW complex,resulting in decreased expression of BrrGL2,a positive regulator of trichomes development.In contrast,AtTCP4 is known to regulate trichomes development by interacting with AtGL3 in Arabidopsis thaliana.Overall,this study revealed that BrrTCP4b is involved in trichome development by interacting with BrrTTG1 in turnip,indicating a divergence from the mechanisms observed in model plant A.thaliana.The findings contribute to our understanding of the regulatory mechanisms governing trichome development in the non-model plants turnip.
基金项目
Strategic Priority Research Program of the Chinese Academy of Sciences,Pan-Third Pole Environment Study for a Green Silk Roa(XDA2004010306)
Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502)
Science and Technology Program of Xizang Autonomous Region(XZ202001ZY0003G)
国家科技期刊平台
NSTL
万方数据