首页|Salicylic acid positively regulates maize defenses against lepidopteran insects

Salicylic acid positively regulates maize defenses against lepidopteran insects

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In response to insect attack,plants use intricate signaling pathways,including phytohormones,such as jasmonate(JA),ethylene(ET),and salicylic acid(SA),to activate defenses.Maize(Zea mays)is one of the most important staple food crops around the world.Previous studies have shown that the JA and ET signaling play important roles in maize defense against insects,but little is known about whether and how SA regulates maize resistance to insect herbivores.In this study,we ectopically expressed the NahG(salicylate hydroxylase)gene in maize plants(NahG maize)to block the accumulation of SA.It was found that compared with the wild-type(WT)maize,the NahG maize exhibited decreased resistance to the generalist insects Spodoptera litura and Spodoptera frugiperda and the specialist Mythimna separata,and the compromised resistance in the NahG maize was associated with decreased levels of defensive me-tabolites benzoxazinoids(Bxs)and chlorogenic acid(CA).Quantification of simulated S.litura feeding-induced JA,JA-isoleucine conjugate(JA-Ile),and ET in the WT and NahG maize indicated that SA does not regulate JA or JA-Ile,but positively controls ET.We provide evidence suggesting that the SA pathway does not crosstalk with the JA or the ET signaling in regulating the accumulation of Bxs and CA.Tran-scriptome analysis revealed that the bHLH,ERF,and WRKY transcription factors might be involved in SA-regulated defenses.This study uncovers a novel and important phytohormone pathway in maize defense against lepidopterous larvae.

BenzoxazinoidsSalicylic acidHerbivoryMaizeTranscriptome

Yohannes Besufekad Setotaw、Jing Li、Jinfeng Qi、Canrong Ma、Mou Zhang、Cuilian Huang、Lei Wang、Jianqiang Wu

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Department of Economic Plants and Biotechnology,Yunnan Key Laboratory for Wild Plant Resources,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China

CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,China

State Key Laboratory of Plant Diversity and Specialty Crops,Beijing 100093,China

国家自然科学基金国家自然科学基金Postdoctoral Fellowship Program of CPSFYunnan Revitalization Talent Support Program"Yunling Scholar"ProjectYunnan Innovation Team Project中国科学院战略规划重点项目"西部之光"人才培养计划General and Key Project of Applied Basic Research Program of YunnanSpecial Research Assistant of Chinese Academy of SciencesPostdoctoral Directional Training Foundation of Yunnan Province

U23A2019932302464GZC20232764202105AE160013XDPB16202201AS070053

2024

10.1016/j.pld.2024.03.004

植物多样性(英文)
中国科学院昆明植物研究所，中国植物学会

植物多样性(英文)

CSTPCD
影响因子：0.617
ISSN：2096-2703
年,卷(期)：2024.46(4)
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