首页|Nigella sativa protects against isoproterenol-induced myocardial infarction by alleviating oxidative stress, biochemical alterations and histological damage

Nigella sativa protects against isoproterenol-induced myocardial infarction by alleviating oxidative stress, biochemical alterations and histological damage

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Objective:To evaluate the cardioprotective effect of Nigella sativa L.(N.sativa) in isoproterenol-induced myocardial infurction (MI).Methods:Groups were treated with different doses of ethanol extract of N.sativa (EENS)and N.sativa oil alone and along with enalapril for 28 days.MI was induced by subcutaneous administration of isoproterenol (85 mg/kg) in two consecutive doses.Levels of cardiac biomarkers and antioxidant enzymes such as creatine kinase-N-acetyl-L-cysteine,lactate dehydrogenase,aspartate aminotrmsferasc,malondialdehyde,supcroxide dismutase,reduced glutathione and catalase were evaluated along with gross histopathological examination.Results:lsoproterenol (85 mg/kg) induced MI by causing the significant (P ＜ 0.01)reduction in the activity of cardiac biomarkers (creatine kinase-N-acetyl-L-cysteine,lactate dehydrogenase,aspartate aminotransferase) and antioxidant markers (superoxide dismutase,catalase,glutathione) along with significant (P ＜ 0.01) increase in the level of malondialdehyde.Furthermore,histopathological evaluation also confirmed the isoproterenol-induced MI.Pretreatment with EENS (800 mg/kg) and combination of EENS (800 mg/kg) with enalapril (1 mg/kg) significantly (P ＜ 0.01) prevented the development of these alteration and restored activity of cardiac biomarkers as well as antioxidant markers almost near to normal levels.Histopathological evaluation of cardiac tissue further confirmed the restoration of biochemical activity,Conclusions:Experimental findings thus indicate that EENS (800 mg/kg) demonstrated cardioprotective effect against isoproterenol-induced MI by restoring cardiac biomarkers and antioxidant status.

AntioxidantIsoproterenolMyocardial necrosisNigella sativaOxidative stress

Md.Quamrul Hassan、Mohd.Akhtar、Sayeed Ahmed、Aftab Ahmad、Abul Kalam Najmi

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Department of Pharmacology,Faculty of Pharmacy,Jamia Hamdard(Hamdard University),New Delhi,110062,India

Department of Pharmacognosy,Faculty of Pharmacy,Jamia Hamdard(Hamdard University),New Delhi,110062,India

Health Information Technology Department,Jeddah Community College,King Abdulaziz University,P,O,Box-80283,Jeddah,21589,Kingdom of Saudi Arabia

We thank the University Grants Commission,New Delhi

Grant No.7-268/2009 BSR

2017

亚太热带生物医学杂志(英文版)

亚太热带生物医学杂志(英文版)

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年,卷(期)：2017.7(4)
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