首页|Origanum vulgare L. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney

Origanum vulgare L. leaf extract alleviates finasteride-induced oxidative stress in mouse liver and kidney

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Objective: To investigate the hepatorenoprotective effects of Origanum vulgare L. against finasteride-induced oxidative injury in the liver and kidney of mice. Methods: Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI/MS) analysis was utilized to yield a fingerprint of Origanum vulgare polyphenolic constituents. Thirty BALB/c mice received 0.5 mL/day distilled water, finasteride (25 mg/kg/day for 10 d), and 100, 200, or 400 mg/kg/day finasteride + Origanum vulgare extract with 6 mice per group for five weeks. On day 36, liver and kidney function as well as pro- and anti-inflammatory (IFN-γ, IL-12, IL-6, TNF-α, IL-1β, and IL-10) cytokines were measured. The total antioxidant status, nitric oxide (NO), and malondialdehyde levels as well as the activities of NO synthase and catalase were also evaluated. Histopathological study was conducted to assess the effect of Origanum vulgare extract on finasteride-induced renal and hepatic toxicities. Results: Twenty-five major polyphenolic compounds were identified in the Origanum vulgare extract by LC-ESI/MS. Origanum vulgare extract, especially at 200 and 400 mg/kg/day doses, significantly improved liver and kidney biochemical indices, decreased inflammatory cytokines, increased total antioxidant status and NO synthase and catalase activities, as well as decreased plasma NO and malondialdehyde levels in a dose-dependent manner as compared to the finasteride group. Histopathological results further confirmed the protective effect of Origanum vulgare extract. Conclusions: Origanum vulgare extract ameliorates finasteride-induced hepatic and renal biochemical and histopathological alterations, and restores antioxidant/oxidant balance.

KidneyLiverOriganum vulgare L.InflammationAntioxidant

Qi-Feng Sun、Shi-Xiang Chen、Zhang-Feng Tang、Xiao-Yi Song、Fa Jing、Hao-Tian Wu、Zhong-Yang Ding、Attalla El-kott、Diaa Massoud、Heba Khalifa

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Department of Hepatobiliary and Vascular Surgery,Jinan City People's Hospital,Jinan,Shandong Province,271100,China

Department of Hepatology,Zhejiang University Ming Zhou Hospital,Ningbo,Zhejiang Province,315199,China

Department of General Surgery,Wuxi Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Traditional Chinese Medicine,Wuxi,Jiangsu Province,214023,China

Biology Department,College of Science,King Khalid University,Abha,Saudi Arabia

Zoology Department,College of Science,Damanhour University,Damanhour,Egypt

Biology Department,College of Science,Jouf University,P.O. Box 2014,Sakaka,Al-Jouf,Saudi Arabia

Zoology Department,Faculty of Science,Fayoum University,Fayoum,Egypt

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King Khalid University,Abha,KSA through Research Group Project under grant number R.G.P.2/80/41 and Wuxi Municipal Health and Fa

WXQ201832

2021

10.4103/2221-1691.311755

亚太热带生物医学杂志(英文版)

亚太热带生物医学杂志(英文版)

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年,卷(期)：2021.11(5)
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