首页|Ethyl acetate fraction of Sargassum pallidum extract attenuates particulate matter-induced oxidative stress and inflammation in keratinocytes and zebrafish

Ethyl acetate fraction of Sargassum pallidum extract attenuates particulate matter-induced oxidative stress and inflammation in keratinocytes and zebrafish

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Objective:To evaluate the effect of the ethyl acetate fraction derived from Sargassum pallidum extract against particulate matter(PM)-induced oxidative stress and inflammation in HaCaT cells and zebrafish.Methods:HaCaT cells and zebrafish were used to evaluate the protective effects of the ethyl acetate fraction of Sargassum pallidum extract against PM-induced oxidative stress and inflammation.The production of nitric oxide(NO),intracellular ROS,prostaglandin E2(PGE2),and pro-inflammatory cytokines,and the expression levels of COX-2,iNOS,and NF-κB were evaluated in PM-induced HaCaT cells.Furthermore,the levels of ROS,NO,and lipid peroxidation were assessed in the PM-exposed zebrafish model.Results:The ethyl acetate fraction of Sargassum pallidum extract significantly decreased the production of NO,intracellular ROS,and PGE2 in PM-induced HaCaT cells.In addition,the fraction markedly suppressed the levels of pro-inflammatory cytokines and inhibited the expression levels of COX-2,iNOS,and NF-κB.Furthermore,it displayed remarkable protective effects against PM-induced inflammatory response and oxidative stress,represented by the reduction of NO,ROS,and lipid peroxidation in zebrafish.Conclusions:The ethyl acetate fraction of Sargassum pallidum extract exhibits a protective effect against PM-induced oxidative stress and inflammation both in vitro and in vivo and has the potential as a candidate for the development of pharmaceutical and cosmeceutical products.

Particulate matterInflammationOxidative stressSargassum pallidumEthyl acetate fractionZebrafish

Wook Chul Kim、Ji-Won Park、Bohyun Yun、WonWoo Lee、Kyung-Min Choi、Seung-Hong Lee

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Department of Medical Science,Soonchunhyang University,Asan 31538,Republic of Korea

Honam National Institute of Biological Resources,99,Gohadoan-gil,Mokpo-si,Jeollanam-do 58762,Republic of Korea

Department of Pharmaceutical Engineering,Soonchunhyang University,Asan 31538,Republic of Korea

Soonchunhyang University Research FundMinistry of Education and National Research Foundation of KoreaKorea Environment Industry&Technology Institute through Project to make multiministerial national biological research resources Korea Ministry of Environment

RS-2023-00230403

2024

10.4103/apjtb.apjtb_921_23

亚太热带生物医学杂志(英文版)

亚太热带生物医学杂志(英文版)

ISSN：
年,卷(期)：2024.14(4)
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