首页|Noscapine shows antimalarial activity against Plasmodium falciparum 3D7,its clinical isolate Pf140/SS,and Plasmodium berghei ANKA

Noscapine shows antimalarial activity against Plasmodium falciparum 3D7,its clinical isolate Pf140/SS,and Plasmodium berghei ANKA

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Objective:To evaluate the antimalarial activity of noscapine against Plasmodium falciparum 3D7 strain(Pf3D7),its clinical isolate(Pf140/SS),and Plasmodium berghei ANKA(PbA).Methods:Using ring-stage survival assay,phenotypic assessments,and SYBR-green-based fluorescence assay,the antimalarial activities of noscapine were assessed compared with dihydroartemisinin(DHA)in in vivo and in vitro studies.In addition,hemolysis and cytotoxicity tests were carried out to evaluate its safety.RT-PCR assay was also conducted to determine the effect of noscapine on papain-like cysteine protease Plasmodium falciparum falcipain-2(PfFP-2).Results:The antimalarial efficacy of noscapine against Pf3D7 and Pf140/SS was comparable to DHA,with IC50 values of(7.68±0.88)and(5.57±0.74)nM/mL,respectively,and>95%inhibition of PbA infected rats.Noscapine also showed a safe profile,as evidenced by low hemolysis and cytotoxicity even at high concentrations.Moreover,PfFP-2 expression was significantly inhibited in both noscapine-treated Pf3D7 and Pf140/SS(P<0.01).Conclusions:Noscapine has antimalarial properties comparable to standard antimalarial DHA with better safety profiles,which may be further explored as a therapeutic candidate for the treatment of malaria.

MalariaPlasmodium falciparumPlasmodium bergheiNoscapineAntimalarialDihydroartemisininCytotoxicityFalcipain-2

Swaraj Kumar Babu、Sameer Maharana、Satyaranjan Chhatria、Dibya Ranjan Sahoo、Ashirbad Nanda、Satish Kanhar、Prativa K.Behera、Sanjib Mohanty、Pradeep Kumar Naik、Praveen Kishore Sahu

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Centre of Excellence in Natural Products and Therapeutics,Department of Biotechnology and Bioinformatics,Sambalpur University,Jyoti Vihar,Sambalpur Odisha,India

Department of Molecular Biology and Infectious Diseases,Community Welfare Society Hospital,Rourkela,Odisha,India

Molecular&Immunology Laboratory,Infectious Disease Biology Unit,Ispat General Hospital,Rourkela,Odisha,India

Department of Pharmacy,Centurion University of Technology and Management,Bhubaneswar,Odisha,India

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2024

10.4103/apjtb.apjtb_342_24

亚太热带生物医学杂志(英文版)

亚太热带生物医学杂志(英文版)

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年,卷(期)：2024.14(8)
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