首页|新疆布尔根河狸国家级自然保护区狭耳鼠耳蝠线粒体基因组研究

新疆布尔根河狸国家级自然保护区狭耳鼠耳蝠线粒体基因组研究

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为提供鼠耳蝠属(Myotis)蝙蝠类遗传及进化分子水平的证据,通过高通量测序法对新疆布尔根河狸国家级自然保护区栖息的狭耳鼠耳蝠(M.blythii)线粒体基因组全序列进行测定和分析.结果显示:狭耳鼠耳蝠线粒体基因组总长为16 752 bp,总碱基组成为33.8%A、30.4%T、22.7%C和13.1%G;线粒体基因组由13个蛋白质编码基因、2个rRNA(12S和16S)基因、22个tRNA基因和1个非编码控制区组成;控制区基因长度为948 bp,比大鼠耳蝠(M.myotis)等姊妹物种短很多;与从NCBI下载的其他鼠耳蝠属蝙蝠线粒体基因组序列的系统发育分析比较,狭耳鼠耳蝠与大鼠耳蝠亲缘关系最近.研究结果丰富了鼠耳蝠属线粒体基因组信息,明确了新疆狭耳鼠耳蝠在鼠耳蝠属内的系统进化地位和亲缘关系,为蝙蝠科(Vespertilionidae)系统分子进化和后续研究提供基础数据.
Study on the Mitochondrial Genomes of Myotis blythii in the Buergen Beaver National Nature Reserve,Xinjiang
To provide molecular level evidence for the genetics and evolution of bats belonging to the genus Myotis,this study used high-throughput sequencing to determine and analyze the mitochondrial genome sequence of M.blythii,a spe-cies inhabiting at the Buergen Beaver National Nature Reserve of Xinjiang,China.Results showed that the total length of the mitochondrial genome of M.blythii was 16,752 bp,and the total base groups were 33.8%A,30.4%T,22.7%C,and 13.1%G.The mitochondrial genome consisted of 13 protein coding genes,2 rRNAs(12S and 16S RNA)genes,22 tRNA genes,and a noncoding control region.The length of the control region gene was 948 bp,which was much shorter than that of sister species such as M.myotis.Based upon mitochondrial genome sequences collected from NCBI for phyloge-netic analysis of other bat species belonging to the genus Myotis,the analysis showed that M.blythii and M.myotis had the closest genetic relationship.The research findings of this study have enriched the mitochondrial genome information of this species,clarified the phylogenetic status and phylogenetic relationship of M.blythii in Xinjiang within the genus Myotis,and provided a data basis for the systematic and molecular evolution of Vespertilionidae and subsequent research.

Mitochondrial genomesMyotis blythiiEndangered speciesHigh throughput sequenc-ingPhylogeny

阿斯姑丽·阿布都热合曼、初雯雯、戚英杰、初红军、吴红潘、何承斌

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新疆大学生命科学与技术学院,乌鲁木齐,830046

新疆林科院森林生态研究所,乌鲁木齐,830002

新疆维吾尔自治区草原总站,乌鲁木齐,830049

北京林业大学生态与自然保护学院,北京,100083

新疆阿尔泰地区自然保护协会,阿勒泰,836500

汕头市濠江区农业农村局乡村振兴发展中心,汕头,515071

新疆布尔根河狸国家级自然保护区管理局,阿勒泰,836500

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线粒体基因组 狭耳鼠耳蝠 濒危物种 高通量测序 系统发育

科技部新疆综合科学考察专项(第三次)科技部新疆综合科学考察专项(第三次)国家林业和草原局科学技术司林草科技创新发展研究项目新疆维吾尔自治区国际科技合作项目

2021xjkk12002021xjkk1201202213201720136026-2

2024

10.12375/ysdwxb.20240106

野生动物学报
东北林业大学 中国动物园协会

野生动物学报

北大核心
影响因子：0.271
ISSN：1000-0127
年,卷(期)：2024.45(1)
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