Analysis of Gut Microbiota Diversity of Tarim Red Deer in Winter Based on the SMRT Sequencing
The Tarim red deer(Cervus elaphus yarkandensis)is the only subspecies of red deer that has adapted to the desert habitat in China.However,there is limited knowledge about how the gut microbiota's structure and function of wild Tarim red deer adapted to desert plants.This study analyzed the gut microbiota of 25 fecal samples obtained from Tarim red deer located in the Upper Tarim River Nature Reserve using the PacBio single molecule real-time sequencing(SMRT)technique.A total of 298,578 clean reads were obtained and clustered into 28,026 OTU,belonging to 25 phyla and 684 genera.Alpha diversity analysis yielded Shannon index of(7.764±0.044),Simpson index of(0.015±0.001),Chao1 in-dex of(12,242.668±695.106),and ACE index of(9,366.423±407.612).The dominant phyla in the gut microbiota of Tarim red deer were Firmicutes(60.51%),Bacteroidetes(26.02%),Proteobacteria(3.61%),Verrucomicrobia(1.57%),and Planctomycetes(1.46%).At the genus level,Papillibacter(5.48%),Bacteroides(5.12%),Muribaculum(4.69%),Phocaeicola(4.49%),Lawsonibacter(4.31%),Ruminococcus(4.09%),Intestinimonas(3.06%),Prevotella(2.87%),Christensenella(2.71%),and Campylobacter(2.54%)were the top 10 bacteria.PICRUSt analysis revealed that the pre-dicted gene functions of the Tarim red deer gut microbiota were mainly in metabolism(48.58%),genetic information pro-cessing(19.04%),and environmental information processing(12.08%).Carbohydrate metabolism(9.47%)and amino acid metabolism(9.93%)were the main categories of metabolism,while membrane transport(9.96%)was the most en-riched gene category of environmental information processing.This study provided the first insight into the characteristics and functions of the gut microbiota of wild Tarim red deer,which may serve as a basis for wild population conservation.
Tarim red deer(Cervus ela-phus yarkandensisGut microbiotaSingle molecule real-time sequencingPICRUSt function predic-tion
NETL
NSTL
万方数据
维普
