高浓度褪黑素对麝鼠香腺中FGF20基因表达的影响
Effect of High Concentration of Melatonin on FGF20 Gene Expression in Muskrat Scent Glands
刘德旺 1白素英1
作者信息
- 1. 东北林业大学野生动物与自然保护地学院,哈尔滨,150040
- 折叠
摘要
麝鼠(Ondatra zibethicus)是一种具有较高经济价值的香料动物和毛皮动物.在一些毛皮动物生产中常使用褪黑素(melatonin,MT)来获得更高的效益,但MT对麝鼠香腺发育的作用尚不清楚,为了探究高浓度MT对麝鼠香腺中FGF20基因表达的影响,通过RT-qPCR技术检测高浓度MT处理组与对照组麝鼠香腺中FGF20基因的转录水平.结果显示:FGF20基因在对照组中的表达水平为高浓度MT处理组(每只麝鼠埋植1粒含有20 mg MT的埋植剂)的33.95倍,二者差异极显著(p<0.01).由此推测,FGF20基因参与了香腺发育的调控,并且高浓度的MT抑制了FGF20基因的表达,研究结果可以为探究适量使用MT提高麝鼠的繁殖力和泌香产量提供依据.
Abstract
Muskrat(Ondatra zibethicus)is a spice and fur animal with high economic value.Melatonin(MT)is commonly used in the production of some fur-bearing animals for higher benefits,but the role of MT on the development of muskrat scent glands is un-clear.In order to explore the effect of high concentration MT on the expression of fibroblast growth factor 20(FGF20)gene in muskrat's gland,RT-qPCR technique was used to detect the transcription level of FGF20 gene in muskrat gland of high concentration MT treatment group and control group.The results showed that the ex-pression level of FGF20 gene in the control group was 33.95 times higher than that in the high concentration MT treatment group(each muskrat was implanted with one capsule containing 20 mg MT),and the difference was extremely significant(p<0.01).It is speculated that FGF20 gene is involved in the regulation of the development of scent gland,and a high con-centration of MT inhibits the expression of FGF20 gene.The results of this study provide a basis for exploring the improve-ment of fertility and fragrance production of muskrats with appropriate use of MT.
关键词
麝鼠/香腺/褪黑素/FGF20基因Key words
Muskrat(Ondatra zibethi-cus)/Scent gland/Melatonin/FGF20 gene引用本文复制引用
基金项目
国家级大学生创新训练项目(202310225293)
出版年
2024
NETL
NSTL
万方数据