Analysis of the Destructive Intensity of D-type Botulinum Neurotoxin by Gastrointestinal Contents of Different Harmful Rodents in Plateau Pastoral Areas
In order to investigate the relationship between the damage intensity by different gastrointestinal contents to the botulinum toxin type D and the sensitivity of the rodents to the botulinum toxin type D of rats in plateau grazing area,the me-dian lethal dose(LD50)of botulinum toxin type D to Ochotona curzoniae,Eospalax baileyi and Neodon fuscus were deter-mined by Hohn's method.The toxin residues of three kinds of gastrointestinal contents and their supernatant after interaction with type D botulinum toxin were determined.The results showed that the LD50 of type D botulinum toxin to Ochotona cur-zoniae,Eospalax baileyi,and Neodon fuscus were 5,110,5,840,and 50,100 MLD/kg,respectively.The damage intensity of the order of the stomach and intestinal contents to the toxin was Neodon fuscus,Eospalax baileyi,and Ochotona curzoniae.The gastric and intestinal contents of the three kinds of rodents had different destructive effects on botulinum toxin type D,and there was a positive correlation between LD50 and toxin residue.The difference of gastric and intestinal environment is one of the reasons for the difference of sensitivity of different harmful rodents to botulinum toxin type D.The result of this study is of significance for the future selection and breeding of efficient and specific botulinum toxin type D biological rodenticide.
Rodent damage in grass-landBotulinum toxin type DIntestine contentsBreaking strengthQinghai-Xizang Plateau pa-storal area
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万方数据
