A New Amphibian Distribution Record of Nidirana guibeiensis in Hunan Province
During the amphibian survey in Langshan Town(26°19′40″ N,110°42′54″ E;altitude 441 m),Xinning County,Hunan Province,China on 26 May,2023,four Nidirana specimens were collected.We compared the specimens with the known congeners and found that the specimens from Hunan were similar to Nidirana guibeiensis.We further con-structed the phylogenetic tree based on COI gene and calculated the genetic distances between species of the genus Nidirana used Kimura 2-parameter model.The phylogenetic tree showed that the specimens from Hunan were clustered with the N.guibeiensis with a high support(1.00/99).The genetic distance between the speci-mens from Hunan and N.guibeiensis from type locality was 0,which was much lower than that between Nidirana species range from 2.7%to 14.4%.Based on morphological and molecular comparisons,the specimens were identified as N.gui-beiensis,which is a new record of Hunan Province,China.
NidiranaNidirana guibeiensisNew recordHunan Province
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