Preliminary Investigation of Potential Pathogens of Sika Deer in Taohongling,Jiangxi Based on Metagenomics
In recent years,positive vegetation succession is obvious in Jiangxi Taohongling Sika Deer National Nature Re-serve,and a large number of sika deer(Cervus nippon)begin to spread and gather in the periphery of the reserve.To in-vestigate the potential pathogen carrying situation of the sika deer in the reserve is helpful to grasp the health status of its population,so as to formulate precise conservation and management countermeasures.We collected the fresh fecal samples of sika deer from different zones of the reserve(core zone,buffer zone and experimental zone)and analyzed the pathogen carring status using metagenomic sequencing technology.The results showed that the sika deer in Taohongling Reserve carried 63 species of pathogenic microorganisms in 34 genus,including bacteria,fungi,actinomycetes,mycoplas-mas,chlamydia and spirochetes,totaling 63 species in 34 genus,and most of which were zoonotic pathogens.The five ge-nus with the highest abundance of pathogens were Clostridioides,Listeria,Klebsiella,Shigella,and Salmonella,and the five species with the highest abundance were Clostridioides difficile,Listeria monocytogenes,Klebsiella pneumoniae,Sal-monella enteritidis,and Shigella flexneri.The number of pathogens carried by sika deer in the core zone was significantly lower than that in the buffer zone and experimental zone.Our results indicated that the sika deer in Taohongling Reserve carry a variety of pathogens,with a certen risk of epidemics occurrence.It is recommended to carry out habitat dwarfing in the core zone,and at the same time,to carry out continuous monitoring and early warning of high-risk pathogens.In addi-tion,conservation management of the proliferating sika deer population should be strengthened.
万方数据
维普
