Effects of Different Nutrient Composition on Intestinal Flora of Chipmunks
In order to investigate the effects of main food nutrients on intestinal flora structure of chipmunks(Tamias sibiri-cus),this study used field food as a control and artificially prepared feed to feed chipmunks.Through fecal sampling,16S rRNA high-throughput sequencing was used to determine and study the changes of intestinal flora composition of chip-munks.According to the different food sources and the content of fat and starch in the feed,the fed chipmunks were di-vided into field group,fat group,starch group and feed group.The results showed that the most abundant bacterial phyla found in chipmunk guts were Firmicutes,Bacteroidetes,and Proteobacteria.The two dominant genera were Muribacula-ceae_norank and Ligilactobacillus.The diversity analysis showed that the species abundance and diversity of intestinal flora in the field group were highest.The intestinal flora structure of the two groups was comparable in between the field and the starch group.Correlation analysis showed that crude protein content was positively linked with the presence of Firmicutes.Starch and crude fibre content were positively correlated with Bacteroidetes,whereas crude fat level was inversely corre-lated with Bacteroidetes.The presence of Actinobacteria was positively related to the amount of crude protein contents.The above results suggested that the changes in food composition affected the composition,structure and richness of intes-tinal flora of chipmunks and that there was significant correlation between food composition and specific intestinal flora.
万方数据
维普
