Effects of Thermal Acclimation on Oxidative Stress and Antioxidant Defense Systems in the Nanorana parkeri
In this study,Nanorana parkeri,an amphibian endemic to the Tibetan Pla-teau,was treated with thermal acclimation at 26 ℃(16 ℃ as the control)for two weeks in the laboratory to investigate the effects of long-term thermal acclimation on oxidative stress level and antioxidant defense system of N.parkeri.The results show that the content of oxidized glutathione in muscle tis-sues(t=1.4,df=10,p＜0.001)and the ratio of oxidized to reduced glutathione in liver and muscle tissues were signifi-cantly increased due to the thermal acclimation,suggesting an enhanced oxidative stress.The activity of superoxide dis-mutase,catalase,glutathione peroxidase,and glutathione S-transferase showed a tissue-specific increase.Moreover,malondialdehyde content decreased significantly in the liver but did not change in muscle tissues,suggesting that thermal acclimation did not lead to an increase in the indicator of oxidative damage,which was attributed to a well-established anti-oxidant defense system.This study will help to elucidate the effects of global warming on the amphibians living on the Ti-betan Plateau.
万方数据
维普
