野生动物学报2024,Vol.45Issue(3) :670-674.DOI:10.12375/ysdwxb.20240325

朱鹮性别分子鉴定的研究进展

Advances in Molecular Identification of Gender in Crested Ibis

邱国强 杜美衡 白洪青 刘小甜 杨永春 宋厚辉 牛冬
野生动物学报2024,Vol.45Issue(3) :670-674.DOI:10.12375/ysdwxb.20240325
✕

朱鹮性别分子鉴定的研究进展

Advances in Molecular Identification of Gender in Crested Ibis

邱国强 1杜美衡 2白洪青 3刘小甜 1杨永春 2宋厚辉 2牛冬2
扫码查看

作者信息

  • 1. 德清县生态林业综合服务中心,德清,313200
  • 2. 浙江农林大学动物科技学院,动物医学院,杭州,311300
  • 3. 德清县自然资源和规划局,德清,313200
  • 折叠

摘要

朱鹮(Nipponia nippon)为世界濒危物种,也是国家一级重点保护鸟类.虽然一系列的保护措施显著增加了朱鹮种群规模和分布区域,但近交衰退、低繁殖力和雏鸟高致残率等问题一直制约着朱鹮种群的发展.朱鹮由于雌雄同型,难以从外观区分性别,因此性别鉴定将对其人工繁殖具有重要意义.本文综述了朱鹮性别的分子鉴定方法,主要包括基于性染色体连锁的染色体解旋酶DNA结合蛋白CHD基因及EcoR Ⅰ片段的PCR鉴定方法,以期进行有效的人工繁育工作,实现对朱鹮种群的保护及规模重建.

Abstract

The crested ibis(Nipponia nippon)is a globally endangered species and holds the status of being a national first-class key protected bird in China.Although a series of conservation measures have significantly increased the population size and distribution area of this species,its population growth has been restricted by inbreeding depression,low fecundity and high rate of nestling disability.It is difficult to distinguish the gender of crested ibis from its appearance because of ho-motype of male and female.Therefore,the gender identification in crested ibis holds great significance for captive breeding efforts.This paper reviews the current research progress in molecular methods for gender determination in crested ibis,fo-cusing primarily on PCR techniques based on the gender chromosome-linked chromodomain-helicase-DNA binding protein gene CHD and EcoR Ⅰ fragment,with an objective to facilitate effective captive breeding and contribute to the conservation and population recovery of the crested ibis.

关键词

朱鹮/性别鉴定/CHD基因/EEO.6序列/分子鉴定

Key words

Crested ibis(Nipponia ni-ppon)/Sex determination/CHD gene/EE0.6 sequence/Molecular identification

引用本文复制引用

基金项目

德清县生态林业综合服务中心项目(ZJXRDQ-2023-DT02)

出版年

2024
野生动物学报
东北林业大学 中国动物园协会

野生动物学报

北大核心
影响因子:0.271
ISSN:1000-0127
参考文献量5
段落导航相关论文