矿化细菌Bacillus paramycoides对重金属Pb(Ⅱ)和Cd(Ⅱ)的去除及矿化特性
Removal and Mineralization of Heavy Metals Pb(Ⅱ)and Cd(Ⅱ)by Mineralizing Bacterium Bacillus Paramycoides
初诗禹 1王瀚瑛 1黎再权 1肖旭 1徐涛 1王慧 1蒋科1
作者信息
- 1. 贵州民族大学 生态环境工程学院,贵阳 550003
- 折叠
摘要
微生物诱导生物矿化可有效固定重金属离子,被认为是一种重要的重金属污染环境修复工艺,对重金属污染生态修复有重要的现实意义.从贵州黔西北某铅锌矿区周边土壤中分离出一株耐 Pb(Ⅱ)和 Cd(Ⅱ)菌株 GS-2,经形态学特征和16 S rRNA鉴定为Bacillus paramycoides,菌株GS-2 对Pb(Ⅱ)和Cd(Ⅱ)的耐受分别高达 1 500 和 50 mg/L,对 250 mg/LPb(Ⅱ)和 5 mg/L Cd(Ⅱ)去除率分别为 99.54%和 69.04%,对重金属Pb(Ⅱ)和Cd(Ⅱ)(均为 25 mg/L)混合去除率分别为 99.73%和71.46%.SEM-EDS、FTIR和 XRD结果显示,菌株通过表面的羟基、羧基、酰胺基和磷酸基团等官能团将 Pb(Ⅱ)和 Cd(Ⅱ)诱导矿化成 Ca2.5 Pb7.5(OH)2(PO4)6、Ca2.1 Pb7.9 Cl2(PO4)6、Pb3(PO4)2、Pb5(PO4)3 OH、Pb9(PO4)6、Pb10(PO4)6(OH)2 和Ca0.67 Cd0.33 CO3 等晶体矿物.菌株 GS-2 具有较好的去除和矿化能力,对环境中 Pb(Ⅱ)和Cd(Ⅱ)污染的生物修复具有一定应用潜力.
Abstract
Microbial induced biomineralization can effectively immobilize heavy metal ions,which is considered to be an important process for the environmental remediation of heavy metal pollution,and has important practical significance for the ecological remediation of heavy metal pollution.In this study,a strain GS-2 resistant to Pb(Ⅱ)and Cd(Ⅱ)was isolated from the soil of a lead-zinc mining area in northwest Guizhou Province.It was identified as Bacillus paramycoides.Based on morphological characteristics and 16 S rRNA.The removal rates of 250 mg/L Pb(Ⅱ)and 5 mg/L Cd(Ⅱ)were 99.54%and 69.04%,respectively,and the removal rates of heavy metal Pb(Ⅱ)and Cd(Ⅱ)(both 25 mg/L)were 99.73%and 71.46%,respectively.The results of SEM-EDS,FTIR,and XRD showed that the strain-induced mineralization of Pb(Ⅱ)and Cd(Ⅱ)to Ca2.5 Pb7.5(OH)2(PO4)6,Ca2.1 Pb7.9 Cl2(PO4)6,Pb3(PO4)2,Pb5(PO4)3 OH,Pb9(PO4)6,Pb10(PO4)6(OH)2 and Ca0.67 Cd0.33 CO3 through the functional groups of hydroxyl,carboxyl,amide and phosphate groups on the surface.Strain GS-2 has great removal and mineralization ability and has certain application potential for bio-remediation of Pb(Ⅱ)and Cd(Ⅱ)pollution in the environment.
关键词
Bacillus/paramycoides/生物吸附/生物矿化/重金属/作用机理Key words
Bacillus paramycoides/biosorption/biomineralization/heavy metals/mechanism of action引用本文复制引用
基金项目
国家自然科学基金资助项目(41907218)
中国博士后科学基金项目(2021MD703822)
贵州省科技厅自然科学基金项目(ZK[2021]235)
贵州省科技厅自然科学基金项目(ZK[2021]209)
出版年
2024
NETL
NSTL
万方数据