首页|Spatiotemporal variations of sand hydraulic conductivity by microbial application methods

Spatiotemporal variations of sand hydraulic conductivity by microbial application methods

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The spatiotemporal distributions of microbes in soil by different methods could affect the efficacy of the microbes to reduce the soil hydraulic conductivity.In this study,the specimens of bio-mediated sands were prepared using three different methods,i.e.injecting,mixing,and pouring a given microbial so-lution onto compacted sand specimens.The hydraulic conductivity was measured by constant-head tests,while any soil microstructural changes due to addition of the microbes were observed by scan-ning electron microscope(SEM)and mercury intrusion porosimetry(MIP)tests.The amount of dextran concentration produced by microbes in each type of specimen was quantified by a refractometer.Results show that dextran production increased exponentially after 5-7 d of microbial settling with the supply of culture medium.The injection and mixing methods resulted in a similar amount and uniform dis-tribution of dextran in the specimens.The pouring method,however,produced a nonuniform distri-bution,with a higher concentration near the specimen surface.As the supply of culture medium discontinued,the dextran content near the surface produced by the pouring method decreased dramatically due to high competition for nutrients with foreign colonies.Average dextran concentration was negatively and correlated with hydraulic conductivity of bio-mediated soils exponentially,due to the clogging of large soil pores by dextran.The hydraulic conductivity of the injection and mixing cases did not change significantly when the supply of culture medium was absent.

Bio-mediated soilDextranHydraulic conductivityLeuconostoc mesenteroidesMicrobial applicationMicrostructure

Viroon Kamchoom、Thiti Khattiwong、Treesukon Treebupachatsakul、Suraparb Keawsawasvong、Anthony Kwan Leung

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Excellent Centre for Green and Sustainable Infrastructure,Department of Civil Engineering,School of Engineering,King Mongkut's Institute of Technology Ladkrabang(KMITL),Bangkok,10520,Thailand

Department of Biomedical Engineering,School of Engineering,King Mongkut's Institute of Technology Ladkrabang(KMITL),Bangkok,10520,Thailand

Department of Civil Engineering,Thammasat School of Engineering,Thammasat University,Prathumthani,10200,Thailand

Department of Civil and Environmental Engineering,The Hong Kong University of Science and Technology(HKUST),Hong Kong,China

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King Mongkut's Institute of Technology Ladkrabang(KMITL)National Science,Research and Innovation Fund(NSRF)Climate Change and Climate Variability Research in Monsoon Asia(CMON3)from the National Research Council of Thailand(NRCT)National Natural Science Foundation of China(NSFC)Hong Kong Research Grants Council

FRB66065/0258-RE-KRIS/FF66/53N10A650844GRF16207521

2024

10.1016/j.jrmge.2023.04.024

岩石力学与岩土工程学报(英文版)
中国科学院武汉岩土力学所中国岩石力学与工程学会武汉大学

岩石力学与岩土工程学报(英文版)

CSTPCD
影响因子：0.404
ISSN：1674-7755
年,卷(期)：2024.16(1)
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