首页|A new bacterial concentration method for large-scale applications of biomineralization

A new bacterial concentration method for large-scale applications of biomineralization

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Bacterial suspension is an essential component of microbially induced carbonate precipitation(MICP)-based biocement and a large-scale production is required for field applications.In this study,a new bacterial concentration method is proposed to enable high concentration bacterial suspension to be produced to facilitate field work.By adding low concentration calcium to bacterial suspension,flocs are formed and bacterial cells are adsorbed on the flocs to achieve bacterial concentration.Compared to the traditional bacterial concentration method using centrifugation and freezing-drying method,the pro-posed method can concentrate a large volume of bacterial suspension without using special equipment.The feasibility of this method is verified by bacterial concentration tests,solution tests and sand column treatment tests.The results of both the solution test and the sand column treatment test show that the bacterial suspension concentrated by the proposed method can be effectively used for soil bio-cementation.There is a threshold calcium concentration that allows a complete bacterial concentration for the proposed method,and this threshold calcium concentration tends to increase linearly with the optical density of the cell suspension at a wavelength of 600 nm(OD600).

BiocementBiocementationBacterial concentrationMicrobially induced carbonate precipitation(MICP)

Hanjiang Lai、Xingzhi Ding、Mingjuan Cui、Junjie Zheng、Jian Chu、Zhibo Chen、Jianwei Zhang

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Zijin School of Geology and Mining,Fuzhou University,Fuzhou,350108,China

Fujian-Taiwan Science and Technology Cooperation Base of Fujian Province on Intelligent Geo-Environmental Engineering,Fuzhou,350116,China

College of Civil Engineering,Fuzhou University,Fuzhou,350108,China

School of Civil Engineering,Wuhan University,Wuhan,430072,China

School of Civil and Environmental Engineering,Nanyang Technological University,Singapore

School of Civil Engineering and Architecture,Henan University,Kaifeng,475004,China

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2024

10.1016/j.jrmge.2024.01.015

岩石力学与岩土工程学报(英文版)
中国科学院武汉岩土力学所中国岩石力学与工程学会武汉大学

岩石力学与岩土工程学报(英文版)

CSTPCD
影响因子：0.404
ISSN：1674-7755
年,卷(期)：2024.16(12)