首页|Effect of acid type on biomineralization of soil using crude soybean urease solution

Effect of acid type on biomineralization of soil using crude soybean urease solution

扫码查看
原文链接
  • 万方数据
  • 维普
The one-phase-low-pH method is a simple,efficient,and user-friendly biogrouting technique that can effectively improve the biomineralization of enzyme-induced carbonate precipitation(EICP)using free urease enzyme.One of the most significant advantages of this method is its capacity to effectively delay calcium carbonate(CaCO3)precipitation by reducing the pH of the solution through the addition of acid.This prevents bioclogging during the biogrouting process and improves the biomineralization effect.However,the biomineralization of the one-phase-low-pH based EICP method may be influenced by the specific acid used.To investigate the impact of acid type on the one-phase-low-pH EICP method using crude soybean urease solution(CSUS),four types of acids,including hydrochloric acid(HCl),nitric acid(HNO3),acetic acid(CH3COOH),and lactic acid(C3H6O3),were used to adjust the pH of CSUS.A series of macroscopic and microscopic experiments were conducted to evaluate the effect of acid type on the one-phase-low-pH EICP method.The results indicate that the acid has an inhibition on the urease activity(UA)of CSUS.Among the acids tested,HNO3 exhibits the most pronounced inhibitory effect on the UA of CSUS,followed by HCl,and the least pronounced inhibitory effect for CH3COOH and C3H6O3 under the same pH conditions.Meanwhile,CH3COOH and C3H6O3 could provide a longer delay duration of CaCO3 precipitation than HNO3 and HCl.Therefore,the one-phase-low-pH EICP method based on CH3COOH and C3H6O3 can significantly improve the effective biocementation depth compared to that based on HNO3 and HCl.Nevertheless,the different types of acids appear to have no obvious effect on the polymorph and crystalline of the precipitated CaCO3 crystals.

Enzyme-induced carbonate precipitation(EICP)One-phase-low-pH methodAcid typeCrude soybean urease solution(CSUS)Biomineralization

Yajie Weng、Hanjiang Lai、Junjie Zheng、Mingjuan Cui、Yihang Chen、Zhitao Xu、Wensong Jiang、Jianwei Zhang、Yu Song

展开 >

School of Civil and Hydraulic Engineering,Huazhong University of Science and Technology,Wuhan,430074,China

Zijin School of Geology and Mining,Fuzhou University,Fuzhou,350108,China

School of Civil Engineering,Wuhan University,Wuhan,430072,China

College of Civil Engineering,Fuzhou University,Fuzhou,350116,China

School of Civil and Architecture Engineering,Henan University,Kaifeng,475004,China

School of Civil Engineering,Guilin University of Technology,Guilin,541006,China

展开 >

2024

10.1016/j.jrmge.2024.09.017

岩石力学与岩土工程学报(英文版)
中国科学院武汉岩土力学所中国岩石力学与工程学会武汉大学

岩石力学与岩土工程学报(英文版)

CSTPCD
影响因子：0.404
ISSN：1674-7755
年,卷(期)：2024.16(12)