评价快速碳青霉烯酶灭活试验在肠杆菌科细菌碳青霉烯酶表型检测中的应用价值
Evaluation of the value of a rapid carbapenemase inactivation method for the phenotypic detection of carbapenemases in Enterobacteriaceae
孙文悦 1周小梅 1卢金英 1梁栋伟1
作者信息
- 1. 广东省人民医院南海医院检验科,广东佛山 528200
- 折叠
摘要
目的 评价快速碳青霉烯酶灭活试验(rCIM)与改良碳青霉烯灭活试验(mCIM)在肠杆菌科细菌碳青霉烯酶表型检测中的应用价值.方法 选择86株耐碳青霉烯肠杆菌科细菌,使用聚合酶链式反应(PCR)检测常见碳青霉烯酶基因,比较rCIM与mCIM法检测碳青霉烯酶表型的性能.结果 共检测到61株碳青霉烯酶基因型,检出率为70.9%.mCIM法发现阳性菌株61株,阴性菌株25株,rCIM法发现阳性菌株62株,阴性菌株24株.以PCR结果作为金标准,mCIM法的准确度、灵敏度和特异度分别为100.0%、100.0%和100.0%,rCIM法的准确度、灵敏度和特异度分别为98.8%、100.0%和96.0%.结论 rCIM法检测肠杆菌科细菌碳青霉烯酶表型,操作简便,不需过夜培养,不需特殊的仪器或试剂,准确度较高.
Abstract
Objective To evaluate the application value of rapid carbapenemase inactivation method(rCIM)and modified carbapenem inactivation method(mCIM)in the phenotypic detection of carbapenemases in Enterobacteriaceae.Methods 86 strains of carbapenem resistant Enterobacteriaceae were selected.The common carbapenemase genes were detected by Polymerase chain reaction(PCR).The performance of rCIM and mCIM in detecting carbapenemase phenotype was compared.Results A total of 61 carbapenemase genotypes were detected,with a detection rate of 70.9%.The mCIM test found 61 positive strains and 25 negative strains,while the rCIM test found 62 positive strains and 24 negative strains.With PCR results as the gold standard,the coincidence rate,sensitivity and specificity of mCIM test were 100.0%,100.0%and 100.0%,respectively,and those of rCIM test were 98.8%,100.0%and 96.0%,respectively.Conclusion The rCIM assay detects the carbapenemase phenotype of Enterobacteriaceae bacteria,which is easy to operate,doesn't require overnight culture,doesn't require special instruments or reagents,and has high accuracy.
关键词
肠杆菌科/碳青霉烯酶/碳青霉烯酶灭活试验Key words
Enterobacteriaceae/Carbapenemase/Carbapenemase inactivation method引用本文复制引用
基金项目
佛山市卫生健康局医学科研课题(20230231)
出版年
2024
NETL
NSTL
万方数据