Determination of Thiourea and Thiourea Dioxide in Environmental Water Sample by High Performance Liquid Chromatography
This work aims to develop a method based on high performance liquid chromatography(HPLC)for determination of thiourea and thiourea dioxide simultaneously in environmental water sample.The chromato-graphic conditions are optimized,and mobile phase is 1 mL·min-1 of acetonitrile-pure water solution(5∶95,V/V).Diode array detector is used and the wave length varies with time,and the detection wave lengths of thi-ourea and thiourea dioxide are set at 237.3 and 271.1 nm,respectively.The external standard method is used for quantitative analysis,and the standard curves have been established for thiourea(0～5.0 mg·L-1)and thiourea dioxide(0～50.0 mg·L-1),exhibiting good linearity with correlation coefficients over 0.999.The collected surface water and tap water samples are centrifuged,and the supernatants are injected into a HPLC system for analysis.Detection limits of thiourea and thiourea are 0.01 and 0.1 mg·L-1,and limits of quantifi-cation are 0.03 and 0.33 mg·L-1,respectively.The recoveries of water samples spiked with thiourea and thi-ourea dioxide are all over 94.9%with RSD ranging from 0.2%to 1.2%,indicating accuracy,stability,and reliability of the method,which will meet the requirement of environmental water analysis.
thioureathiourea dioxidehigh performance liquid chromatographyenvironmental water sample
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