Aiming at the problem of spontaneous mutation of Aspergillus oryzae in the process of industrial brew-ing,A.oryzae X3042 and JN1 that used commonly in factories was studied in this paper.The hydrolase activity was determined after separating pure single colony and solid fermentation culture,and then the new strains of X3042-4 and JN1-3 were selected by rejuvenation.The protease,cellulase,amylase and lipase activity of A.oryzae X3042-4 increased 22.37%,10.91%,15.23%and 27.71%respectively compared with the initial strain A.oryzae X3042,and the protease,cellulase,amylase and lipase activity of A.oryzae JN1-3 increased 10.71%,2.88%,7.81%and 7.17%respectively compared with the initial strain A.oryzae JN1.In addition,due to the amylase activity is slightly low,the new strain of A.oryzae JN1-3-4 was obtained by ultraviolet mu-tagenesis,and the amylase activity was 2.69 times that of initial strain JN-1-3.Furthermore,the protease,cel-lulase and lipase activity also had a certain increase.This study provided a technical reference about rejuve-nation and mutation breeding of fermentation strains.
关键词
米曲霉/菌种复壮/诱变育种/发酵培养/水解酶系
Key words
Aspergillus oryzae/rejuvenation/mutation breeding/fermental cultivation/hydrolase series
维普
万方数据